Can cell lines be maintained in culture?
Many continuous mammalian cell lines can be maintained on a relatively simple medium such as MEM supplemented with serum, and a culture grown in MEM can probably be just as easily grown in DMEM or Medium 199. However, when a specialized function is expressed, a more complex medium may be required.
What is a human cell culture line?
Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid. Cell culture studies provide greater understanding in the areas of developmental biology, protein and gene expression, as well as genetic evolution. …
What happens if cells are over confluent?
When the cells are approximately 80% confluent (80% of surface of flask covered by cell monolayer) they should still be in the log phase of growth and will require sub-culturing. (Do not let cells become over confluent as they will start to die off and may not be recoverable).
How do you authenticate a cell line?
The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.
What does ATCC mean?
American Type Culture Collection
ATCC (American Type Culture Collection) is a private, nonprofit, global biological resource center and standards organization that provides scientists with the biomaterials and resources they need to conduct critical life science research.
Why do we use ATCC strains?
That’s why ATCC scientists have collected, authenticated, and preserved microorganisms for nearly a century to ensure that researchers have access to the strains needed to develop vaccines and therapeutics, clean up oil spills, ensure healthier food and water, and develop products to protect our health and well-being.
Are HeLa cells still used?
The immortality of HeLa cells contributed to their adoption across the world as the human cell line of choice for biomedical research. Though additional cells lines have been developed over the years, HeLa cells continue to be widely used to advance biomedical research and medicine.