Is luciferase a plasmid?
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.
What is pGL3?
Description. The pGL3 Luciferase Reporter Vectors(a) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors.
What is pGL3 basic vector?
Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.
Can plasmids be manipulated?
Importantly, because the bacteria from which plasmids are isolated grow quickly and make more of the plasmids as they grow, scientists can easily make large amounts of plasmid to manipulate and use in later work.
Is a reporter a plasmid?
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. For bacteria or prokaryotic cells in culture, this is usually in the form of a circular DNA molecule called a plasmid.
Are plasmids inherited?
Plasmids with different origins of replication and different replication genes are able to inhabit the same bacterial cell and are considered compatible (left). During cell division, both types of plasmid replicate; therefore, each daughter cell will inherit both plasmids, just like the mother cell.
What is a reporter plasmid?
What is a reporter tag?
The use of epitope tags in recombinant DNA techniques allows the detection of proteins where specific antibodies are not available. Reporter genes (also known as protein expression markers or small reporter proteins) are used in gene expression studies and provide an indication of the transcriptional activity in cells.