How does caffeine affect onion root tip?

How does caffeine affect onion root tip?

If onion root cells are immersed in water with any concentration of caffeine, then root growth, and therefore mitosis, will be inhibited because it affects cell plate formation.

How do you make a slide out of onion root tip?

An Alternative Procedure

1. Cut the tip 5 to 8 mm from the tip of the freshly sprouted root.
2. Place the cut tip on a clean microscope slide.
3. Add 2-3 drops of acetocarmine stain to the slide.
4. Warm the slide gently over the alcohol lamp for about one minute.
5. Cover the slide with a cover slip or lens paper.

Why does a slide prepared with onion root tip need to be thin?

An onion root tip is a rapidly growing part of the onion and thus many cells will be in different stages of mitosis. The onion root tips can be prepared and squashed in a way that allows them to be flattened on a microscopic slide, so that the chromosomes of individual cells can be observed easily.

What affects the speed of mitosis?

Growth & Repair The rate of mitosis must increase in order to produce these new cells. Similarly the rate of mitosis also increases during periods of growth, such as our development in the womb, childhood and puberty. In pants there is also much higher rates of mitosis during germination and growth.

Does caffeine stimulate mitosis?

Overall, most research data indicate that caffeine does indeed reverse DNA-damage-induced G2 arrest and drive cells through a fatal mitosis. Many reports suggested that the mechanism may be related to caffeine’s ability to reactivate Cdc2 and Cdc25C activities.

Why we use HCL in onion root tip?

1. The root tip is heated with acid to break up the tissues into individual cells. The cellulose walls of plant cells are held together by pectins such as calcium pectate. Treatment with hydrochloric acid breaks this down.

How do you make onion root tips grow faster?

Place the onions in a clear glass or jar with the root end down. Add just enough water to the bottom of the container to cover the roots at the bottom of the bulbs. Change the water at least every other day. Place the glass in a window that receives plenty of filtered sunlight throughout the day.

How do you make onion roots grow faster?

Why is an onion root tip good for studying mitosis?

Why is onion root tip used to demonstrate mitosis in this experiment? It is because of the meristematic cells that are situated in the tip of the roots that render the most desirable and suitable raw material to study the different stages of mitosis. Onion is a monocot plant. Hence, their root tips are used.

What magnification do you need to see mitosis?

Focusing the microscope with 40x objective should give you a close enough view of the chromosomes to find each phase. You will also look for spindle fibers which are attached to each chromosome and are used by the cell to separate the chromosomes and move them to each pole.

What tissue has the highest rate of mitosis?

Skin cells
Skin cells, hair follicles and the cells lining our intestines (epithelial cells) all have high rates of mitosis as these tissues constantly need to be replaced. In plants growth occurs largely at the shoot and root tips. These cells have much higher rates of mitosis than the rest of the plant.

What can you see in an onion root tip cell?

When observing the onion root tip cells for the stage of prophase, the cells took on a brick-like structure and within the cells, small dots (the nuclei) can be seen. In one particular cell’s nucleus, the chromatin has condensed so much that it can be seen using a light microscope. The stage that the cell is currently in is prophase.

Where does chromatin line up in an onion root cell?

When observing the whitefish embryo cells for the stage of metaphase, the cells took on a circular shape and, like the onion root cell, many nuclei can be seen. In a certain cell, the chromatin, that was condensing during prophase, line up at the equatorial plate. That certain cell is in the metaphase stage.

How are whitefish embryos like onion root cells?

When observing the whitefish embryo cells for the stage of metaphase, the cells took on a circular shape and, like the onion root cell, many nuclei can be seen. In a certain cell, the chromatin, that was condensing during prophase, line up at the equatorial plate.