Can DAPI be frozen?
you can aliquot your stock solution and freeze it at -20°C as it is already mentioned. The used aliquot you can store in the fridge, do not freeze it again. You can dilute DAPI in the last rinsing buffer or directliy in the last anti-body solution.
How long is the incubation period for DAPI?
2. Add sufficient 300 nM DAPI stain solution to cover the cells. 3. Incubate for 1–5 minutes, protected from light.
Do you wash after DAPI?
Image the samples; washing is optional but not required. Note: Samples can be stored at 4°C after staining and before imaging. Note: DAPI can be included directly in antifade mounting medium for one-step mounting and staining.
How do you dissolve DAPI on PBS?
First you make a stock solution of 1 mg/ml (dissolved in water or PBS) and then you dilute the solution 1:5000. If you are left with a colloidal liquid, leave if for 5 to 10 min in 60 degrees heated water bath. You can also use sonication and heating to better help the dissolving process.
How do you store DAPI?
The 5 mg/mL DAPI stock solution may be stored at 2–6°C for up to 6 months or at ≤–20°C for longer periods.
How is DAPI stored?
When stored at +15 to +25°C, the product is stable through the expiration date printed on the label. 1 DAPI Store at +15 to +25°C. Keep protected from light. Working Solution Concentration Storage and Stability DAPI stock solution 1 to 5 mg/ml Store at −15 to −25°C for 12 months.
How do you dilute DAPI?
Dilute DAPI stock solution to a concentration between 1 – 0.1 µg/ml in PBS and incubate for 5 min at room temperature in the dark. Rinse samples once in PBS and then prepare for imaging. Examine immediately using appropriate excitation wavelength.
Is DAPI hydrophobic?
Indeed, as DAPI is hydrophobic it confers this behaviour on the DNA and thus restricts the presence of water molecules.