Is AAVS1 a gene?
AAVS1 is an exemplary locus within the PPP1R12C gene that permits robust expression of CAG promoter-driven transgenes.
What is Safe Harbor gene?
Abstract. Genomic safe harbors (GSHs) are sites in the genome able to accommodate the integration of new genetic material in a manner that ensures that the newly inserted genetic elements: (i) function predictably and (ii) do not cause alterations of the host genome posing a risk to the host cell or organism.
Where is AAVS1?
chromosome 19
Adeno-associated virus integration site 1 is a viral integration site that in humans is encoded by the AAVS1 gene located on chromosome 19.
What is the AAVS1 safe harbor locus?
The AAVS1 locus, in intron 1 of PPP1R12C (protein phosphatase 1 regulatory subunit 12C), is known as a “safe harbor” because its disruption does not have adverse effects on the cell, and robust transcription can be used to maintain the expression of an exogenously inserted gene5,6,7.
What is the Rosa26 promoter?
The Rosa26 promoter has even been cloned as a bacterial artificial chromosome (BAC), and harnessed to drive ubiquitous expression of other reporter genes in transgenic mice. The Jackson Laboratory currently distributes over 100 mice that have transgenes inserted in the Gt(ROSA)26Sor locus.
Where is the Rosa26 locus?
mouse chromosome 6
Known in scientific circles as Gt(ROSA)26Sor, the Rosa26 locus is a specific genetic site that is located on mouse chromosome 6, and is considered to be one of the ideal locations where knock-ins of interest can be targeted.
What is the ROSA26 gene?
ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. It was first isolated in 1991 in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 130 knock-in lines have been created based on the ROSA26 locus. The human homolog of the ROSA26 locus has been identified.
What chromosome is ROSA26 on?
chromosome 6
The Rosa26 locus on chromosome 6 is frequently used for the integration of transgene constructs to achieve ubiquitous or conditional gene expression in mice. The Rosa26 transcript is spliced into three exons and ubiquitously expressed in all cell types and developmental stages, but not translated to a protein [1].