What is the specific activity of an enzyme?
Specific enzyme activity (usually stated simply as ‘specific activity’) is the number of enzyme units per ml divided by the concentration of protein in mg/ml. Specific activity values are therefore quoted as units/mg or nmol/min/mg (if unit definition B is applied).
What does a specific enzyme mean?
Enzymes are specific because different enzymes have differently shaped active sites. The shape of the active site of an enzyme is complementary to the shape of its specific substrate . This means they are the correct shapes to fit together. Temperature has an effect on enzyme activity.
What is an enzyme activity assay?
Enzymatic activity assays are predominately performed by researchers to identify the presence or quantity of a specific enzyme in an organism, tissue, or sample. Examples of such enzymes include α-amylase, catalase, laccase, peroxidase, lysozyme, and reporter enzymes alkaline phosphatase, and luciferase.
How do you calculate enzyme activity?
enzyme activity= change in OD/time taken (min) x 1/extinction coefficient of enzyme x total reaction volume/ volume of enzyme extrct taken x total volume of enzyme extract/ Fresh wt of tissue (g) x total protein x 1000 = nano moles of enzyme present per g of sample tissue.
What is meant by specific activity?
Specific activity is the activity per quantity of a radionuclide and is a physical property of that radionuclide. Activity is a quantity (for which the SI unit is the becquerel) related to radioactivity. Thus, specific activity is defined as the activity per quantity of atoms of a particular radionuclide.
What is meant by enzyme specificity quizlet?
Enzyme Specificity. the extent to which an enzyme’s activity is restricted to a specific substrate, a specific group of substrate, a specific type of chemical bind, or a specific type of chemical reaction. Absolute Specificity. catalyze only one reaction.
What determines enzyme specificity?
Specificity is a property of the enzyme and describes how restrictive the enzyme is in its choice of substrate; a completely specific enzyme would have only one substrate. The most important factor governing the fit of a substrate for an enzyme is the amino acid sequence around the bond to be cleaved.
What is the difference between enzyme activity and specific activity?
The main difference between enzyme activity and specific activity is that enzyme activity is the moles of substrate converted by the enzyme per unit time whereas specific activity is the activity of enzyme per milligram of total enzyme.
What is relative enzyme activity?
Relative activity is the ratio between the activity of your sample of interest and the activity of the control sample and therefore expressed as a percentage.
What is specific activity in protein purification?
Specific activity is the ratio of activity units to amount of protein (U/mg), which should increase during the purification. During the purification process, lots of undesired proteins are purified away, but the desired protein giving the activity remains, thereby becoming enriched at each step.
What is the difference between activity and specific activity?
The key difference between enzyme activity and specific activity is that the enzyme activity refers to the amount of substrates converted to products per unit time while the specific activity of an enzyme refers to the activity of an enzyme per milligram of protein.