What is f-12K Medium?
Ham’s F-12K (Kaighn’s) Medium is a modification of Ham’s F-12 Nutrient Mixture. Ham’s F-12K (Kaighn’s) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.
What is kaighn’s modification?
Ham’s F-12K
Ham’s F-12K (Kaighn’s) Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) was originally used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a much lower serum environment.
What is F12 in cell culture?
DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
What is in DMEM F12?
DMEM/F12 Medium is a 1:1 mixture of Dulbecco’s Modified Essential Medium (DMEM) and Ham’s F-12 Medium, commonly used in growing a variety of mammalian cultures. DMEM/F12 is rich and complex, containing all 21 amino acids, 10 vitamins, glucose, zinc, and iron among other components.
What is the difference between DMEM and DMEM F12?
DMEM/F12: It is a mixture of DMEM and Ham’s F-12 and is an extremely rich and complex medium. IMDM is a modification of DMEM containing selenium, and has additional amino acids, vitamins and inorganic salts as compared to DMEM.
What is in Hepes buffer?
This buffer is classified as a zwitterionic sulphonic acid buffering agent with the IUPAC chemical name 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. Its chemical formula can be written as C8H18N2O4S, while its structural formula is written as: HEPES buffer has the following properties: Molar mass: 238.3012 g/mol.
What is composition of DMEM?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What are the components of DMEM?
11965 – DMEM, high glucose
| Components | Molecular Weight | Concentration (mg/L) |
|---|---|---|
| Amino Acids | ||
| Potassium Chloride (KCl) | 75.0 | 400.0 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 3700.0 |
| Sodium Chloride (NaCl) | 58.0 | 6400.0 |
What are the components of Ham’s f-12k medium?
Using Ham’s F-12K Ham’s F-12K (Kaighn’s) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types.
What are the different types of media used in microbiology lab?
Peptone water, nutrient broth and nutrient agar are considered as basal medium. These media are generally used for the primary isolation of microorganisms. Enriched medium (Added growth factors): – Addition of extra nutrients in the form of blood, serum, egg yolk etc, to basal medium makes them enriched media.
How are selective media used for bacterial growth?
Selective media favor the growth of some microorganisms while inhibiting others. Enriched media contain added essential nutrients a specific organism needs to grow Differential media help distinguish bacteria by the color of the colonies or the change in the medium.
Can a microorganism grow in a culture medium?
Culture media contains nutrients and physical growth parameters necessary for microbial growth. All microorganisms cannot grow in a single culture medium and in fact many can’t grow in any known culture medium. Organisms that cannot grow in artificial culture medium are known as obligate parasites.