What is FACSCanto?
The BD FACSCanto™ System is a powerful and reliable cell analyzer for busy, best-in-class laboratories. The BD FACS™ Loader instrument option enables walkaway sample introduction to improve productivity. Unique reflective design improves sensitivity. Innovations in the fluidics system deliver optimal system performance.
How do I claim compensation for a diva?
Select Experiment > Compensation Setup > Calculate Compensation. When the Single Stained Setup box appears, select “Link & Save” to apply the calculated values to your experiment. Load a tube of water on to the instrument and place it in STANDBY. 16.
What is sit in flow cytometry?
When you install tubes onto the sample injection tube (SIT), a pump within the fluidics cart pressurizes the plenum, which then provides sheath fluid to the flow cell. When you remove tubes from the SIT, the cytometer cleans the SIT by flushing sheath solution down the inside and outside of the tube.
What is window extension in flow cytometry?
On some instruments, there is an additional period added to this processing time, called the window extension, on BD instruments. This extension increases the time that the system is looking for a pulse and is depicted in Figure 4. Figure 4: The impact of window extension on pulse processing.
What does events mean in flow cytometry?
In flow cytometry, an “event” is defined as a single particle detected by the instrument. Accurate detection of events using flow cytometry requires the ability to separate single cells with specific characteristics from within a heterogeneous population of cells.
What do my flow cytometry results mean?
Flow cytometry can identify the type of cells in a blood or bone marrow sample, including the types of cancer cells. It detects types of cancer cells based on either the presence or the absence of certain protein markers (antigens) on a cell’s surface.