Can I culture MDA-MB-231 in DMEM?
DMEM is quite a standard and highly recommended medium for growing MDA-MB-231 cells. ATCC uses complete L-15 (+10% FBS).
What media does MDA-MB-231 use?
The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.
What type of breast cancer is MDA-MB-231?
MDA-MB-231 is a highly aggressive, invasive and poorly differentiated triple-negative breast cancer (TNBC) cell line as it lacks oestrogen receptor (ER) and progesterone receptor (PR) expression, as well as HER2 (human epidermal growth factor receptor 2) amplification2,3.
How do you thaw MDA MB 231 cells?
Establishing MDA-MB-231/GFP Cultures from Frozen Cells Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1–2 minutes by gentle agitation in a 37°C water bath.
What are bt474 cells?
Breast Cancer Model BT-474 This expression profile makes them a prevalent model to develop new breast cancer therapeutic agents. BT-474 cells grow in response to estradiol but shows resistance to ER antagonist tamoxifen. Estradiol supplement is required to establish xenograft model of athymic nude mice.
What is the difference between MCF-7 and MDA MB 231?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What type of breast cancer is MCF-7?
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].
What is in F12 media?
Nutrient Mixture F-12 was designed and developed specifically for the growth of Primary Rat Hepatocytes (PRHs) and Rat Prostate Epithelial Cells (RPECs). F-12 is based on Ham’s F-10 medium with increased concentrations of choline, inositol, putrescine and amino acids.
How long does it take for MDA MB 231 cells to adhere?
Just aspirate the media and add more. After this, you should see some cells happily attached. Do not change the media or split cells before they are about 60% confluent (which can take 5-7 days for MDA-MB-231, depending on how many survive thawing), unless there are a lot of dead cells floating.