How do you dissolve bromophenol blue?
Dissolve 5.0 g of bromophenol blue powder (tetrabromophenolsulfonphthalein) in 74.5 mL of 0.1 N sodium hydroxide (NaOH) solution. Dilute with purified water to 500 mL.
What does bromophenol blue bind to?
Bromophenol blue (BPB), a phenolphthalein anionic dye, binds to proteins under neutral to acidic conditions. From this attribute, BPB can determine protein levels in samples, especially in solubilized cultured cells and cell membrane proteins with high concentrations of surfactants (8).
What is the pH of bromophenol blue?
Bromophenol Blue is a pH indicator dye that turns yellow under acidic conditions. Bromophenol Blue has a pI below pH 4.0. The concentrated dye solution may have a reddish or greenish cast to the color. This is normal and expected.
What is bromophenol blue soluble in?
Bromophenol blue is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of bromophenol blue in ethanol is approximately 10 mg/ml and approximately 30 mg/ml in DMSO and DMF.
Is bromophenol soluble?
4-Bromophenol Properties Boiling point: 235-236 °C (lit.) It is insoluble in water. It is soluble in 5%ethanol.
How is bromophenol blue prepared for gel electrophoresis?
Directions:
- Add 25 mg of bromophenol blue to 6.7 ml of ddH2O and mix.
- Add 25 mg of xylene cyanol FF and mix.
- Add 3.3 ml of glycerol and mix.
- Aliquot and freeze at -20 °C for long-term storage.
Is bromophenol blue a pH indicator?
Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB, albutest) is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.
What color is bromophenol blue in a basic solution?
| Indicator | pH Range | Alkali |
|---|---|---|
| 3.0 Benzopurpurin | 1.2-4.0 | red |
| 5.0 Bromocresol green | 3.8-5.4 | blue |
| 6.0 Bromocresol purple | 5.2-6.8 | purple |
| 7.0 Bromophenol blue | 6.0-7.6 | blue |
How do you make blue solution?
Combine 20 mL of alcohol to the sodium hydroxide/bromothymol blue solution. Dilute the mixture with 1 L of distilled water in a large container. The solution should be dark blue in color. If the solution appears green, use a pipette to slowly add sodium hydroxide drop by drop to the solution until it changes to blue.
Which is the correct pH for bromophenol blue?
Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel…
What is the Koc of bromophenol blue?
Using a structure estimation method based on molecular connectivity indices(1), the Koc of bromophenol blue can be estimated to be 1.5X10+6(SRC). According to a classification scheme(2), this estimated Koc value suggests that bromophenol blue is expected to be immobile in soil. The pKa of bromophenol blue is 4.0(3),…
Which is more soluble in water benzene or methyl alcohol?
Soluble in water (about 0.4 g/100 mL); more soluble in methyl and ethyl alcohol, and in benzene. Freely soluble in NaOH solutions with the formation of a water -soluble sodium salt.