What is a organotypic culture?
Organotypic culture is defined as the culture of an organ collected from an organism. It is one method allowing the culture of complex tissues or organs. It allows the preservation of the architecture of the cultured organ and most of its cellular interactions.
What are organotypic slices?
Organotypic brain slice cultures (BSCs) represent a physiologically relevant three-dimensional model of the brain. BSCs support all the central nervous system (CNS) cell types and can be produced from brain areas involved in neurodegenerative disease.
What is Histotypic culture?
Histotypic culture is defined as three-dimensional culture of one cell type, while the term organotypic implies the interaction of two or more cell types from a complex tissue or organ.
What are slice cultures?
Slice cultures grown on filter membranes (Stoppini et al., 1991) provide a means for short- or long-term culturing of brain tissues without the need to physically rotate the tissues or attach them to coverglasses.
What can slice cultures be used for?
Slice Cultures Acute slices are typically used for short-term electrophysiology experiments, while organotypic cultures are used to observe structural and morphological changes such as neuronal migration, axon outgrowth, or synapse formation.
What is an acute brain slice?
Definition. Brain slices are ex vivo preparations obtained by serial sectioning of brain tissue, typically from rats or mice. Acute brain slices are kept vital in vitro for time periods between four and, sometimes, more than twenty-four hours and contain a functional brain cell micro-circuitry in situ.
What is micro carrier culture?
In microcarrier culture, cells grow as monolayers on the surface of small spheres or as multilayers in the pores of macroporous structures that are usually suspended in culture medium by gentle stirring.
What is an organotypic model?
Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic the structure and function of cells and tissues found inside the body.
What are acute slices?
Brain slices are ex vivo preparations obtained by serial sectioning of brain tissue, typically from rats or mice. Acute brain slices are kept vital in vitro for time periods between four and, sometimes, more than twenty-four hours and contain a functional brain cell micro-circuitry in situ.