What is EL4 cell line?
ECACC General Cell Collection: EL4 Cell Line Description: Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.
What is ATCC cells?
ATCC has the world’s largest and most extensive product catalog of human and animal cell lines for research purposes. The cell biology collection includes more than 4,000 continuous cell lines available by species, tissue/disease types, and signaling pathways.
What is EL4 lymphoma?
Abstract. The murine mouse lymphoblastic lymphoma cell line (EL4) tumor model is an established in vivo apoptosis model for the investigation of novel cancer imaging agents and immunological treatments due to the rapid and significant response of the EL4 tumors to cyclophosphamide and etoposide combination chemotherapy …
Which benchmark cell line verification is recommended by ATCC?
To standardize STR analysis for human cell line authentication, the American Tissue Culture Collection (ATCC) Standards Development Organization Workgroup has issued standard ASN-0002, which recommends the use of at least eight STR loci (TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317 and D5S818) plus Amelogenin for …
What is lymphoblastoid?
(LIM-foh-BLAST) A lymphocyte that has gotten larger after being stimulated by an antigen. Lymphoblast also refers to an immature cell that can develop into a mature lymphocyte. Enlarge. Blood cell development.
Which agents does ATCC currently screen cell lines for?
In addition, ATCC stated “As of January 1, 2010, all human cell lines accessioned into the ATCC general collection are tested for the adventitious agents HIV, Hepatitis B, Hepatitis C, HPV, EBV, and CMV.
How does ATCC validate cell lines?
The report meets the requirements for National Institutes of Health funding, journal publication, and quality control for FDA submissions. Unlike other providers, we authenticate cell lines by comparing the STR profile of sample cell lines with the ATCC Human Cell STR Database.
How do you authenticate a cell phone line?
The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.
What is lymphoblastoid cell lines?
Abstract: Lymphoblastoid cell lines (LCLs) represent a convenient research tool for expanding the amount of biologic material available from an individual. LCLs are commonly used as reference materials, most notably from the Genome in a Bottle Consortium.
How can you tell the difference between Myeloblast and lymphoblast?
The main difference between myeloblast and lymphoblast is that the myeloblast differentiates into granulocytes whereas the lymphoblast differentiates into lymphocytes. Furthermore, myeloblast contains granules while lymphoblast does not contain granules.
What kind of cell is EL4 in lymphoma?
EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. The cells are resistant to 0.1 mM cortisol and sensitive to 20 μg/mL PHA. A subline (EL4.IL-2, ATCC TIB-181) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available.
Is the EL4 cell line a negative control?
The CD4 + EL4 thymoma line served as a negative control – transcripts for GAPDH were detected, whereas mRNA for IL-13Rα1 or IL-4Rα was not detected (N.D.). B : NOD splenocytes were stained with antibodies to TCRαβ, CD4, and IL-13Rα1 ( upper plot ) or isotype control ( lower plot ).
How are EL4 cells transfected with Pac-neo-OVA?
The EL4 cells were transfected by electroporation with the plasmid pAc-neo-OVA which carries a complete copy of chicken ovalbumin (OVA) mRNA and the neomycin (G418) resistance gene. E.G7-OVA cells contain a single copy of the inserted plasmid and synthesize and secrete OVA constitutively.
How is ATCC tib-39 used in cancer research?
It also can be used in cell-based assays for cancer research. This luciferase expressing cell line was derived from parental line ( ATCC TIB-39) by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter.