What are the different types of cell lines used?
Understanding Types of Cell Lines
- Frog cell lines.
- Hamster cell lines.
- Mouse cell lines.
- Rat cell lines.
- Dog cell lines.
What is the most commonly used cell line?
HeLa (/ˈhiːlɑː/; also Hela or hela) is an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line.
How many types of cell lines are there?
Cell line popularity can be estimated by the numerous publications using cell lines and American Type Culture Collection (ATCC) Cell Biology Collection which consists of over 3,600 cell lines from over 150 different species.
What are the criteria for choosing cell lines?
There are several key factors that must be considered to ensure that cell lines used in research provide relevant and valid data: correct identity, absence of microbial contamination and stability of characteristics and function over extended in vitro passage.
How do you identify cell lines?
Cell lines can be tested and identified using multiallelic variable number of tandem repeats (VNTR). HLA typing and DNA fingerprinting using short tandem repeat (STR) and a variable number of tandem repeats for intra-species cross-contamination have been used for cell line identification.
How do I choose the right cell line for my research?
The Do’s and Dont’s of Choosing a Cell Line
- Don’t Choose Simply Because It Is Used in the Literature.
- Do Consider If It Fits Your Biological Model.
- Do Consider the Experiments You Want to Perform.
- Table 1: Features of commonly used cell lines.
- Don’t Assume That All Cell Lines Have the Same Culturing Requirements.
What is cell line selection?
The term cell line refers to the propagation of culture after the first subculture. It is possible to select a particular cell lineage by cloning or physical cell separation or some other selection method. Such a cell line derived by selection or cloning is referred to as cell strain.
What are adherent cell lines?
Adherent cells: types of cell lines that grow in the monolayer attached to the surface. While passaged (see standard passaging), a detaching agent (e.g., trypsin) needs to be used to detach them from the surface. They re-attach to the surface within a few hours upon plating.