What is super binary vector?
A super-binary vector carries additional virulence genes from a Ti plasmid, and exhibits very high frequency of transformation, which is valuable for recalcitrant plants such as cereals. A number of useful vectors are widely circulated.
What did binary vector system contain?
Binary system components The T-DNA portion of the binary vector is flanked by left and right border sequences and may include a transgene as well as a plant selectable marker. Outside of the T-DNA, the binary vector also contains a bacterial selectable marker and an origin of replication (ori) for bacteria.
What is pGreen plasmid?
The pGreen plasmids were first described in 2000 as components of a novel T-DNA binary system. As these plasmids have been taken up by the research community, the plasmids have been developed, expanding the resources available to the community.
What is PBIN19?
Explanation: PBIN19 is a disarmed cloning vector which is a binary vector. The left and right borders of this vector flank copy of lacZ’ gene.
What is the binary vector strategy used for?
A binary vector is a standard tool in the transformation of higher plants mediated by Agrobacterium tumefaciens. It is composed of the borders of T-DNA, multiple cloning sites, replication functions for Escherichia coli and A.
What is a binary vector math?
The binary set {0, 1} together with modulo-2 addition and multiplication is called a binary field, which is denoted by F2. A vector space over F2 is called a binary vector space. The set of all binary vectors of length n forms an n-dimensional vector space Vn over F2.
What are the main aspects of plant binary vectors?
Plant Transformation vectors contain three key elements;
- Plasmids Selection (creating a custom circular strand of DNA)
- Plasmids Replication (so that it can be easily worked with)
- Transfer DNA (T-DNA) region (inserting the DNA into the agrobacteria)
What is true about expression vector?
An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell’s mechanism for protein synthesis to produce the protein encoded by the gene.
What is co integrated vector?
Co-integrate vectors are produced by integrating a suitably modified E. coli plasmid containing the gene of interest into a disarmed Ti plasmid. The co-integration of the two plasmids is achieved within the Agrobacterium by homologous recombination.
What is a shuttle plasmid?
A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species. Shuttle vectors include plasmids that can propagate in eukaryotes and prokaryotes (e.g. both Saccharomyces cerevisiae and Escherichia coli) or in different species of bacteria (e.g. both E.