How do you dissolve Hydroxytamoxifen?
I usually dissolve it in absolute ethanol. It may be hard to dissolve so you may want to heat up the samples at 55 c degrees for few minutes to ensure a good solubility.
How do you dissolve 4 Hydroxytamoxifen?
Before use, 4-OHT should be re-dissolved in ethanol by shaking at 37C for 15 min, a 1:4 mixture of castor oil:sunflower seed oil was added to give a final concentration of 10 mg/ml 4-OHT, and the ethanol should be evaporated by vacuum under centrifugation.
How do you make 4 Hydroxytamoxifen?
For injection of 4-OHT, dissolve 100 mg of 4-OHT powder in 1 mL of ethanol (final concentration of 100 mg/mL), and then dilute the solution 1/10 in peanut or corn oil.
Is tamoxifen toxic to mice?
Ways to deliver Tamoxifen to Mice Tamoxifen is a hazardous material – make sure to handle it properly. It is also light sensitive, therefore making fresh solutions is recommended. There are several ways to administer tamoxifen to mice, each with advantages and disadvantages (7):
How do you administer tamoxifen?
Take tamoxifen exactly as directed. Do not take more or less of it or take it more often than prescribed by your doctor. Swallow tamoxifen tablets whole; do not split, chew, or crush them. Swallow the tablets with water or any other nonalcoholic drink.
How do you make tamoxifen solution?
Dissolve 20mg/ml Tamoxifen in corn oil for 1 hr in a roller (hybridization oven) at 65C (protect tube from light with tin foil) Keep your stock at +4C for up to a week. Before injecting, warm Tamoxifen at 65C for 10min.
What does tamoxifen do to mice?
Here, we show that mice undergoing tamoxifen treatment experience changes in temperature, bone, and movement. Single-cell RNA sequencing reveals that tamoxifen treatment induces widespread gene expression changes in the hypothalamus and preoptic area (hypothalamus-POA).
Is tamoxifen soluble in water?
SOLUBILITY / SOLUTION STABILITY: The solubility in water is 0.3 mg/L at 20°C; the pH is approx. 3.0-3.5 at 20°C.
Can tamoxifen be injected?
Administer tamoxifen via intraperitoneal injection (using an ACUC approved injection procedure) once every 24 hours for a total of 5 consecutive days. As a safeguard, sanitize injection site with 70% ethanol prior to injection.
How do you make tamoxifen water soluble?
To deliver tamoxifen in drinking water, dissolve it in pure ethanol (100 mg tamoxifen in 1 mL of ethanol) and then dilute it 100- to 200-fold in distilled water (to a final concentration of 1–0.5 mg/mL).
How do you dissolve tamoxifen in oil?
Dissolve tamoxifen (Sigma‐Aldrich) in corn oil at a concentration of 20 mg/ml by shaking overnight at 37°C. Tamoxifen is light sensitive, and should be made and stored in a light‐ blocking vessel (amber, or foil wrapped).
What does tamoxifen do to your brain?
A team from the University of Rochester Medical Center has shown scientifically what many women report anecdotally: that the breast cancer drug tamoxifen is toxic to cells of the brain and central nervous system, producing mental fogginess similar to “chemo brain.”
What is the working concentration of 4 hydroxytamoxifen?
4-Hydroxytamoxifen is provided in a 13 mM solution. The recommended working concentration is 10-100 μM. Therefore, 4-Hydroxytamoxifen Ready Made Solution should be diluted 1:130 – 1:1,300 in cell culture media.
How is 4-hydroxytamoxifen formed in the liver?
4-Hydroxytamoxifen is an active metabolite of Tamoxifen. 4-Hydroxytamoxifen is formed by cytochrome P450 2D6 in human liver and is a potent and selective estrogen receptor antagonist. 4-Hydroxytamoxifen has been used to stimulate LC3 lipidation and to form autophagic vesicles in a superoxide-dependent manner.
How is 4-hydroxytamoxifen used to treat breast cancer?
4-Hydroxytamoxifen is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants.
Which is better z-4-hydroxytamoxifen or wtcas9?
( Z )-4-Hydroxytamoxifen is a estrogen receptor antagonist. Tamoxifen (Cat. No. 0999) metabolite; exhibits greater potency than the parent compound. Chemotherapeutic agent. Also activates intein-linked inactive Cas9, reducing off-target CRISPR-mediated gene editing; system has ~25-fold higher specificity than wtCas9.