What is bgiseq500?
Abstract. Background: BGISEQ-500 is a new desktop sequencer developed by BGI. Using DNA nanoball and combinational probe anchor synthesis developed from Complete Genomics™ sequencing technologies, it generates short reads at a large scale. We also include examples of the raw images from the sequencer for reference.
How does DNA Nanoball sequencing work?
DNA Nanoball Sequencing involves isolating DNA that is to be sequenced, shearing it into small 100 – 350 base pair (bp) fragments, ligating adapter sequences to the fragments, and circularizing the fragments. The DNA copies concatenate head to tail in a long strand, and are compacted into a DNA nanoball.
What is BGI sequencing?
www.genomics.cn. BGI, currently known as the BGI Group, formerly known as the Beijing Genomics Institute, is a Chinese genome sequencing company, headquartered in Shenzhen, Guangdong, China. It was formed in 1999 to participate in the Human Genome Project as a genetics research center.
What is combinatorial probe anchor synthesis?
Combinatorial probe anchor synthesis (cPAS) The two technologies that form the basis for this high-throughput sequencing technology are DNA nanoballs (DNB) and patterned arrays for nanoball attachment to a solid surface.
How does MGI sequencing work?
MGI’s DNA sequencing instruments utilize the state-of-the-art core technology called DNBSEQTM. DNBs (DNA nanoballs) are pumped with by the fluidics system and loaded onto a Patterned Array chip. This information is then used to determine the DNA sequence of the sample.
What are NGS platforms?
A set of novel sequencing platforms, often collectively named as “next-generation sequencing” (NGS) completely transformed the life sciences, by allowing extensive throughput, while greatly reducing the necessary time, labor and cost of any sequencing endeavor.
What BGI means?
Business Growth Initiative (various organizations) BGI. Borland Graphic Interface (File Name Extension) BGI. Basic Ground Instructor (certification; US FAA)
Is BGI Genomics?
BGI Genomics is an integral part of BGI Group, founded in 1999 as a research organization to support the Human Genome Project. Today, BGI is actively participating in the fields of Cancer Research, Bio-sustainability and Personalized Medicine through a variety of subsidiaries and a global network of collaborators.
Who makes genome sequencing equipment?
DNA sequencer
| DNA sequencers | |
|---|---|
| Manufacturers | Roche, Illumina, Life Technologies, Beckman Coulter, Pacific Biosciences, MGI/BGI, Oxford Nanopore Technologies |
Is SOLiD sequencing still used?
As with the commercialization of automated Sanger sequencing, many of these technologies are no longer in use (for example, Solid™, Polinator™ Helicos™). These “second” generation sequencing technologies and associated methods are described below.
How are nanoball batteries used in the world?
As of right now, nanoball batteries are still in the experimental stage. Besides being used in cars and phones, nanoball batteries could also be used for relief in third-world countries and disaster-stricken areas as their small size and high discharge rates would allow for energy to be quickly and efficiently spread around.
How are the nanoballs used in DNA sequencing?
( c) Ligation-based sequencing chemistry is used to interrogate bases of genomic DNA in the library molecules. Each cycle of sequencing tags the DNA nanoballs with a fluorophore whose color identifies the base (A, C, T or G) present at a specific position.
Why are nanoballs better than bridge amplification 4?
Nanoballs offer a higher array packing density than bridge amplification 4, because they physically exclude other DNA molecules from their spot on the grid, and a much easier and cheaper workflow than emulsion PCR 5, because they are prepared in a simple reaction that does not waste most of the amplification reagents on empty emulsion bubbles.
What kind of material are carbon nanoballs made of?
Most of the carbon nanoballs that was formed were sintered (solid mass of material formed by heat and/or pressure). Trace amounts of nanoballs that existed as individuals rather than a group was also detected as well as a few cotton-like nano-materials.