What is the 100x objective used for?
The total magnification of a low power objective lens combined with a 10x eyepiece lens is 100x magnification, giving you a closer view of the slide than a scanning objective lens without getting too close for general viewing purposes.
Why is immersion oil used in 100x objective?
The 100x lens is immersed in a drop of oil placed on the slide in order to eliminate any air gaps and lossof light due to refraction (bending of the light) as the light passes from glass (slide) → air → glass (objective lens).
Why is oil immersion required when viewing a bacterial smear using 1000x magnification?
Normally, bacteria are in a drop of water contained in a glass slide, and light bends as it leaves the slide. At high magnifications the cone of light must get large — so large that it can miss the lens altogether. That’s where oil immersion comes in.
What is an objective for immersion in the light microscope?
In light microscopy, a water immersion objective is a specially designed objective lens used to increase the resolution of the microscope. This is achieved by immersing both the lens and the specimen in water which has a higher refractive index than air, thereby increasing the numerical aperture of the objective lens.
Why is immersion oil used with the 100x objective quizlet?
Immersion oil has the same refractive index compared with that of glass. This prevents light loss due to diffraction. Oil immersion should be used between the slide and 100x objective lens, this is a special oil that has the same refractive index as glass.
How do you use 100x oil immersion lens?
Place a drop of immersion oil on the top of your cover slip and another drop directly on your 100x oil objective lens. Slowly rotate your 100x oil objective lens into place and adjust the fine focus until you get a crisp and clear image.
How do you use a 100x microscope?
What is the importance of the 100x lens?
The most powerful lens of the light microscope is the 100x oil immersion objective. Because light is refracted every time it passes through a medium with a different refractive index, (air to glass or vice versa) the quality of the image is reduced with each passage.
What is the magnification of the 100x objective quizlet?
The distance between the specimen on the stage and the objective lens (how much room you have to move the stage up and down = how much room you have to focus on the image of the specimen). The 100X objective (total magnification when used is 1000X) that is used with immersion oil.
What should I do immediately after using the 100x objective lens?
Slowly rotate your 100x oil objective lens into place and adjust the fine focus until you get a crisp and clear image. When finished viewing with your 100x oil immersion lens, carefully wipe the oil from all glass surfaces using a piece of lens cleaning paper.