What is BCR-ABL international scale?
We aligned BCR-ABL values generated by 38 laboratories to an international scale (IS) where a major molecular response (MMR) is 0.1% or less. This indicates that the IS can deliver accurate comparison of molecular response rates between clinical trials when measured by different laboratories.
What is BCR-ABL negative?
Atypical chronic myeloid leukemia (aCML), BCR-ABL1 negative is a rare myelodysplastic syndrome/myeloproliferative neoplasm for which no current standard of care exists. The blood smear of patients with aCML showed prominent immature granulocytosis, and granulocytic dysplasia.
What type of protein is BCR-ABL?
A protein made from pieces of two genes that get joined together. It is found in most patients with chronic myelogenous leukemia (CML), and in some patients with acute lymphoblastic leukemia (ALL) or acute myelogenous leukemia (AML).
How does a BCR-ABL work?
The increased tyrosine kinase activity of p210BCR/ABL results in phosphorylation of several cellular substrates and in autophosphorylation of p210BCR/ABL, which in turn induces recruitment and binding of a number of adaptor molecules and proteins.
Is there an international scale for Bcr-Abl p190 testing?
Current testing options for BCR-ABL p190 are not only cumbersome, but also expensive and time consuming for the lab to implement because: No international scale has been established for p190 monitoring, so laboratories are dependent on ratios and must run laborious standard curves.
How is GeneXpert performed in a Cepheid cartridge?
The cornerstone of the GeneXpert testing process is Cepheid’s patented, self-contained, single use cartridge. Sample extraction, amplification and detection are all carried out within this self-contained “laboratory in a cartridge”.
What are the features of the Xpert BCR-ABL ultra?
Based on the innovative GeneXpert technology, Xpert BCR-ABL Ultra automates the entire test process including RNA isolation, reverse transcription, and fully nested real-time PCR of BCR-ABL target gene and ABL reference gene in one fully automated cartridge.
Which is the best BCR-ABL minor breakpoint assay?
Xpert BCR-ABL Ultra p190 is a quantitative assay for the BCR-ABL minor breakpoint (p190) transcripts that provides high assay sensitivity, standardization, and on-demand molecular results in about 2.5 hours.