Who invented acid-fast stain?
Paul Ehrlich is the first to describe the acid-fast properties of the bacterium. In the 1890s, Friedrich Neelsen and Franz Ziehl modified the stain by adding phenol (carbolic acid) and basic fuschin.
When was acid-fast staining developed?
1882
Acid fast staining was developed in Ehrlich in 1882 and was later successively modified by Ziehl and Neelsen. Its main application is in the staining and demonstration of acid fast bacilli in sputum smears.
What is the theory behind acid-fast staining?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
What is the purpose of an acid-fast stain?
Acid fast stains are used to differentiate acid fast organisms such mycobacteria. Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the Kinyoun stain.
What is acid-fast staining in microbiology?
Acid fastness is a physical property that gives a bacterium the ability to resist decolorization by acids during staining procedures. This means that once the bacterium is stained, it cannot be decolorized using acids routinely used in the process.
Why is bacilli called acid-fast?
Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast.
What are the basic characteristics of acid-fast staining technique?
The acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium . Acid-fast organisms are characterized by wax-like, nearly impermeable cell walls; they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.
What is the primary stain used in acid fast staining?
Carbolfuchsin
Ziehl Neelsen Acid-fast stain
| ACID-FAST STAIN | Cell Color | |
|---|---|---|
| Procedure | Reagent | Acid-fast Bacteria |
| Primary dye | Carbolfuchsin | RED |
| Decolorizer | Acid-alcohol | RED |
| Counterstain | Methylene blue | RED |
Why is bacilli acid-fast?
Acid-fast bacillus (AFB) is a type of bacteria that causes tuberculosis and certain other infections. Tuberculosis, commonly known as TB, is a serious bacterial infection that mainly affects the lungs. It can also affect other parts of the body, including the brain, spine, and kidneys.
What is the principle of acid-fast bacilli?
The mycobacterial cell wall contains mycolic acids, which are fatty acids that contribute to the characteristic of “acid-fastness.” The principle of the AFB smear is based on the fact that mycolic acid in the cell wall of AFB render them resistant to decolorization with acid alcohol.
What is the process of acid fast staining?
Procedure of Acid-Fast Stain Prepare bacterial smear on clean and grease free slide, using sterile technique. Allow smear to air dry and then heat fix. Cover the smear with carbol fuchsin stain. Heat the stain until vapour just begins to rise (i.e. about 60 ). Wash off the stain with clean water.
What is the purpose of an acid – fast stain?
Acid-Fast (AF) is an important special staining technique used in the histology lab. This is adifferential stain used to identify acid-fast bacterial organisms, such as members of the genus Mycobacterium and Nocardia .
What disease are diagnosed using acid fast stain?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis ( TB) and other illnesses. Your health care provider will collect a sample of urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection.
Why is acid fast staining necessary?
Acid fast stains are used to differentiate acid fast organisms such mycobacteria. Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the Kinyoun stain.