How do you predict DNA binding domain?
If you have full sequence of amino acid of your protein or gene sequence of your protein then you can predict DNA binding site or DNA binding domain. It is depended on source of protein. If structure of protein (pdb) is knownthen your task begun easy.
What are DNA binding motifs in proteins?
The DNA binding proteins have a specific folding pattern which is made of repeated nucleotide sequences called as DNA binding motifs. These motifs permit the binding of DNA binding proteins such as transcription factors to the target sequence. It is composed of recognition region and stabilization region.
What is DNA sequencing motif?
Sequence motifs are short, recurring patterns in DNA that are presumed to have a biological function. Often they indicate sequence-specific binding sites for proteins such as nucleases and transcription factors (TF).
Which item is a common DNA binding motif?
Among the most common DNA-binding domains are the zinc finger domains, helix-turn-helix, leucine zipper, homeobox domains, and basic helix-loop-helix structure.
Why are DNA-binding proteins important?
DNA-binding proteins have a central role in all aspects of genetic activity within an organism, such as transcription, packaging, rearrangement, replication and repair.
Why is the DNA-binding domain important?
The function of DNA binding is either structural or involves transcription regulation, with the two roles sometimes overlapping. DNA-binding domains with functions involving DNA structure have biological roles in DNA replication, repair, storage, and modification, such as methylation.
What is DNA motif?
A DNA motif is defined as a nucleic acid sequence pattern that has some biological significance such as being DNA binding sites for a regulatory protein, i.e., a transcription factor. DNA motifs are often associated with structural motifs found in proteins. Motifs can occur on both strands of DNA.
What is motif finding problem?
Given a set of DNA sequences (promoter region), the motif finding problem is the task of detecting overrepresented motifs as well as conserved motifs from orthologous sequences that are good candidates for being transcription factor binding sites. A large number of algorithms for finding DNA motifs have been developed.
What is sequence motifs in bioinformatics?
A sequence motif is a nucleotide or amino-acid sequence pattern that is widespread and has, or is conjectured to have, a biological significance. A protein sequence motif is an amino-acid sequence pattern found in similar proteins; change of a motif changes the corresponding biological function.
Which of the following is a major difference between different DNA-binding motifs?
Which of the following is a major difference between different DNA-binding motifs? They employ different mechanisms to position and stabilize a recognition alpha helix in the major groove of DNA.
How does DNA-binding work?
DNA-binding proteins are proteins that attach to DNA. Transcription factors bind to regulatory sequences on DNA and turn transcription of genes on or off. NeuroD1 is an example of a transcription factor needed for neuronal development. It attaches to E-box sequences using the basic helix loop helix domains.