Why are HEK293 cells used for transfection?
Firstly, they are very easy to grow and to maintain, with high reproducibility, which makes them preferable over other less-robust and slow-growing cell lines. Furthermore, they are very efficient at protein production and accessible for transfection.
What is PEI transfection?
DNA can be introduced into a host cell by transfection with polyethylenimine (PEI), a stable cationic polymer (Boussif et al., 1995). PEI condenses DNA into positively charged particles that bind to anionic cell surfaces. Our laboratory uses PEI over other cell transfection reagents because of its low cost.
What is TurboFect?
TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.
What are HEK293 cells used for?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
What is HEK293 cell line?
HEK293 is a hypotriploid human cell line. The modal HEK293 chromosome number is 64 in 30% of cells, with higher ploidy occurring in 4.2% of cells. They have three copies of the X chromosome, and a 4 kbp fragment of Ad5 was incorporated into chromosome 19 of the HEK293 genome, which displays cytogenetic instability.
Where do HEK 293 cells come from?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
How to optimize the HEK293 transfection protocol?
Optimizing Transfection: To obtain the highest transfection efficiency, optimize transfection conditions by varying HEK293 cell density and amount of transfection reagent. High passage of HEK293 cells and use of antibiotics (or growth factors) may require using larger volumes of HEK293 transfection reagent per reaction. Limited Use Label License:
Which is the best transfection reagent for HEK 293 cells?
TransIT®-293 Reagent Achieves High Transfection Efficiency. HEK 293 cells transfected with pEGFP using Trans IT®-293 Reagent in complete media for 24 hours.
Which is the best reagent for RNAi transfection?
Lipofectamine LTX Reagent performs well with vector-based RNAi experiments. For Invitrogen™ siRNA and Stealth RNAi™ transfections, we recommend Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent.