What is MALDI-TOF mass spectrometry?
Matrix-Assisted Laser Desorption/Ionization-Time Of Flight (MALDI-TOF) mass spectrometry (MS) is a common method used for quality control (QC) of oligonucleotides. The MALDI-TOF instrument uses pulses of laser light to vaporize the oligo/matrix in a process known as desorption.
What is MALDI-TOF used for?
Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.
What molecule does MALDI-TOF identify?
2.1. MALDI is an ionization technique in which a matrix absorbs energy from ultraviolet lasers to create ions from large molecules with minimal fragmentation. The m/z ratio can be determined by the TOF of the ions which the detector measure TOF of ions to calculating masses of ions (Fig.
What is the function of a matrix in MALDI-TOF MS analysis?
In MALDI technology, the matrix has a special effect on the analysis of the sample: dilute the sample to dissociate the clustered macromolecule; protect the sample, the matrix absorbs the laser energy and then transfers it to the sample to avoid direct laser irradiation of the sample and lead to decomposition of the …
What is the function of Maldi-Tof in determining the protein composition and structure?
The MALDI-TOF can be used in profiling and imaging proteins directly from thin tissue sections, known as MALDI imaging mass spectrometry (MALDI-IMS). It provides specific information about the local molecular composition, relative abundance and spatial distribution of peptides and proteins in the analyzed section.
What types of sample are suitable for Maldi-TOF mass analysis?
Samples for MALDI analysis may be supplied either in solution or lyophilised. Ideally samples should be resuspended in an aqueous solution of 50% (v/v) acetonitrile, 0.1% (v/v) trifluoroacetic acid using ultra high purity water. If any other solution is used, BioCentre staff must be supplied with comprehensive details.
What is the principle of MALDI-TOF What is its main use in protein studies?
As shown in Figure 2, the basic principle of TOF is that ions of different m/z are dispersed in time during their flight along a field-free drift path of known length.
What types of molecules are the best ones to use for MALDI-TOF?
A number of organic compounds have been used as matrices for MALDI-TOF MS but for microbiological applications, α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxy benzoic acid (DHB), and 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid) have been found to be the most useful.
What is the principle of Maldi-Tof What is its main use in protein studies?