What is Illumina nextera?
Nextera XT DNA Library Preparation Kit (24 samples) Each Nextera XT DNA Library Preparation kit provides reagents sufficient for preparing small genomes, PCR amplicons, and plasmids sequencing-ready libraries for up to 24 samples, with a low DNA input requirement.
How does Illumina Tagmentation work?
Tagmentation is the initial step in library prep where unfragmented DNA is cleaved and tagged for analysis. Bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization steps.
What is Illumina TruSeq?
TruSeq DNA Library Preparation Kits are a simple, cost-effective solution for generating high-quality libraries, compatible with unparalleled Illumina sequencing output. Each index has been rigorously tested to ensure high performance across all Illumina sequencing platforms.
What is Tagment DNA?
What is Tagmentation? Tagmentation is the initial step in library prep where unfragmented DNA is cleaved and tagged for analysis. Bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization steps.
What is the purpose of a tailing before adding the adapters?
Generally, a single adenine base is added to form an overhang via an A-tailing reaction. This A overhang allows adapters containing a single thymine overhanging base to base pair with the DNA fragments.
Can a NextEra kit be used with an Illumina sequencer?
Samples prepared with Nextera kits are compatible with all Illumina sequencers. With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.
Which is the best NextEra DNA prep kit?
The Nextera DNA Library Prep Kit has been obsolesced. For whole-genome sequencing, Nextera DNA Flex Library Prep Kit is the recommended replacement product.
What are the IDT for Illumina NextEra DNA sets?
The products previously known as IDT for Illumina Nextera DNA Unique Dual Indexes Sets A and B (Cat. No. 20027213 and 20027214) are now called IDT for Illumina DNA/RNA UD Indexes Sets A and B. The index sequences and kit configurations have not changed.
How many samples are in the Nextera XT Kit?
Multiplexing of up to 384 samples per Nextera XT library is available for projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing.