Can 7-AAD be used with APC?
As for compensation controls, please use drug-treated cells stained with either Annexin V-APC or 7-AAD. 3. Annexin V-APC can be detected in APC channel while 7-AAD can be detected in PerCP/Cy5.
Can I use PerCP and PerCP Cy5 5 together?
Anyways, the two dyes cannot be used simultaneously as there is overlap in their emission spectra. And so you got the spectral overlap. As these two dyes are tandem dyes and in both of them the reciepient dye is cy5, they could not be used simultanously.
Can you use APC and PE-Cy5 together?
For example, PE-Cy5 is very bright with regard to stain index (Table 1); but it has considerable spillover into the APC detector. While these two fluorochromes can be used together, the resolution sensitivity in APC will be reduced compared to, for example, a combination of PerCP-Cy5.
Does PE and FITC overlap?
In some experiments FITC may be combined with other dyes, for example PE, that emit yellow and orange photons. Fig. FITC spillover into other channels. FITC single stained lymphocytes show spillover into PE and PE-Cy5 detectors.
Is BV480 a tandem dye?
Due to its emission profile, BV480 has less spillover into the BV605, BV650 and BV711 channels and, in general, is brighter than BV510. 405 nm 610/20 BD Horizon Brilliant™ Violet 605 (BV605) (Exmax 407 nm/Emmax 602 nm) is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 602 nm.
How do you compensate FACS?
How To Compensate A 4-Color Flow Cytometry Experiment Correctly
- 4 Steps To Compensating A 4-Color Experiment.
- Choose the correct carrier for compensation.
- Step 2: Collect the data and make sure there is a sufficient number of events.
- Calculate compensation correctly.
- Apply the compensation values and inspect the results.
Are PerCP and PerCP Cy5 5 the same?
PerCP-Cy5. 5 (Ex-Max 482 nm/Em-Max 695 nm): is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5. 5 is not subject to photobleaching like PerCP and can be used with stream-in-air flow cytometers.
Can I use FITC to compensate GFP?
Just because GFP, CFSE and FITC are detected in the same channel (FL1), it does not mean you can use them interchangeably when setting up your compensation. GFP, CFSE and FITC are unique flourochromes and have different spillover into other channels.
Does FITC bleed into PE?
where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.
Is BV605 a tandem dye?
405 nm 610/20 BD Horizon Brilliant™ Violet 605 (BV605) (Exmax 407 nm/Emmax 602 nm) is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 602 nm. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates.
Is BV421 a tandem?
(Ex max 407 nm/Em max 786 nm) is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em Max at 786 nm. Across the past 40 years flow cytometry solutions have kept pace with the accelerating speed of discovery in life sciences.