What is SELEX used for?
Systematic Evolution of Ligands by EXponential Enrichment (SELEX) is a common method currently used for isolating high-affinity single-stranded (ss) DNAs or RNAs from a large library with random sequences [1,2,3].
What is SELEX process?
Specifically, SELEX process includes (1) the incubation of target molecules with the random sequence pools, (2) and the subsequent separation of unbound oligonucleotides and the elution of bound oligonucleotides, (3) then PCR amplification of bound aptamers.
What is HT SELEX?
Background. The combination of systematic evolution of ligands by exponential enrichment (SELEX) and deep sequencing is termed high-throughput (HT)-SELEX, which enables searching aptamer candidates from a massive amount of oligonucleotide sequences.
What is counter selection SELEX?
As selection has evolved, researchers have gone beyond negative selection for generic SELEX components and added negative selection (counter selection) steps that actively seek to remove sequences binding to unwanted targets from the oligonucleotide pool.
What does SELEX stand for?
SELEX stands for systematic evolution of ligands by exponential enrichment.
Who invented SELEX?
Professor Larry Gold, and then graduate student Craig Tuerk developed the SELEX process. A randomized pool of around 65,500 nucleotide sequences underwent the process, and two of these sequences were isolated. The experiment’s results led to the concept that nucleic acids could be used to recognize proteins.
Are aptamers cheaper than antibodies?
Currently, a large number of generated aptamers can bind various targets, ranging from simple inorganic molecules to large protein complexes, and entire cells. In fact, aptamers are nucleotide analogues of antibodies, but aptamer-generation is significantly easier and cheaper than the production of antibodies [6, 7].
How do aptamers work?
It works by isolating sequences with increased affinity and specificity to a target molecule from an oligo sequence pool through several rounds of selection. DNA or RNA molecules that bind to the target are prospective aptamers for that target.
Can aptamers bind to DNA?
Aptamers are single-stranded, synthetic oligonucleotides (DNA or RNA) which fold into 3-dimensional shapes capable of binding non-covalently and with high affinity to a target molecule.
Who invented aptamers?
Gold, co-inventor of the technology for making aptamers from nucleic acids, is founder, chairman of the board, and CEO of Soma-Logic, in Boulder, Colo. De Souza is president and CEO of Archemix, in Cambridge, Mass. Between them, these two biotechnology companies own virtually the entire aptamer patent estate.
Can aptamers replace antibodies?
Aptamers can be effectively used in both biotechnology and clinical medicine. They can be beneficial for diagnostic methods in clinical oncology and ophthalmology. Aptamers can replace antibodies in different detection methods. In particular, aptamers are used in an ELISA-based detection assay called ELASA (Figure 3).
What do aptamers do?
Aptamers are single-stranded oligonucleotides that fold into defined architectures and bind to targets such as proteins. In binding proteins they often inhibit protein–protein interactions and thereby may elicit therapeutic effects such as antagonism.
Who are Selex ES and what do they do?
The company delivers robust solutions for the pressing needs of Government Agencies, Armed Forces, and Commercial and Industrial operators. In addition to full design, production, and commissioning in the U.S., Selex ES Inc. ensures seamless access to the full product offerings of the Leonardo Company for military and civil markets.
When did the first SELEX experiment take place?
From the time that the first SELEX experiments were described by 3 independent groups in 1990 the method has been adapted to a wide range of technologies 1-5. A highly multiplexed, parallel HT-SELEX method was developed for NGS. 6 A variation of SELEX-seq 7 uses Nextera adapter sequences for efficient library preparation. 8
Which is a better company Selex ES or Leonardo Company?
Selex ES Inc. proves to be a stronger, more efficient and more flexible company due in large part to the parental support from Leonardo Company, S.p.A.
What is the barcode on a Selex ligand?
Each DNA ligand contains a 14 bp randomized region (14N), and a 5 bp barcode that uniquely identifies the individual SELEX sample. Partially nested primers are used in successive SELEX rounds.