What cells are visible in a blood smear?
The blood smear allows for the evaluation of these cells:
- White blood cells (WBCs, leukocytes) help fight infections or participate in immune responses.
- Red blood cells (RBCs, erythrocytes) carry oxygen to tissues.
- Platelets (thrombocytes) small cell fragments that are vital to proper blood clotting.
What are the types of blood smear?
Exam Overview
- A thick blood smear is a drop of blood on a glass slide. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood.
- A thin blood smear is a drop of blood that is spread across a large area of the slide.
What type of stain is used for blood smears?
Blood films are routinely stained with a Romanowsky-type stain (e.g., Wright or Wright-Giemsa) either manually or using an automatic slide stainer. Romanowsky-type stains are composed of a mixture of eosin and oxidized methylene blue (azure) dyes.
What is the difference between blood film and blood smear?
Left smear is unstained, right smear is stained with Wright-Giemsa stain. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically.
How do you identify cells in a blood smear?
The identification of blood cells is based primarily on observations of the presence or absence of a nucleus and cytoplasmic granules. Other helpful features are cell size, nuclear size and shape, chromatin appearance, and cytoplasmic staining.
What are smudge cells?
Smudge cells are ruptured chronic lymphocytic leukemia (CLL) cells appearing on the blood smears of CLL patients. Our recent findings suggest that the number of smudge cells may have important biologic correlations rather than being only an artifact of slide preparation.
What are the three methods of preparing a blood smear?
Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below.
What is the meaning of PBF?
PBF
Acronym | Definition |
---|---|
PBF | Permanent Bitch Face |
PBF | Positive Balance File (finance) |
PBF | Percentage of Bone Fill |
PBF | Peninsula Bible Fellowship (Bremerton, WA) |
What is the difference between eosinophils and basophils?
Basophils are largely circulating cells, but home to areas of allergic inflammation during the late phase response. Eosinophils are resident to the GI tract, but also home to allergic inflammatory sites.