What does a Nucleofector do?
A Nucleofector® Device that comprises unique electrical parameters pre-programmed for each optimized cell type, to deliver the substrate directly into the cell nucleus and the cytoplasm.
What is Amaxa?
The Amaxa Nucleofector technology is a transfection system designed to transfer genes into cells quickly and efficiently. The technology is based on electroporation. Each cell type is introduced to the DNA (or RNA) in an optimized Nucleofector solution that has been designed for that particular cell type.
What is the difference between Nucleofection and electroporation?
Based on the physical method of electroporation, nucleofection uses a combination of electrical parameters, generated by a device called Nucleofector, with cell-type specific reagents. In contrast, other commonly used non-viral transfection methods rely on cell division for the transfer of DNA into the nucleus.
What is U266?
Also called U266 or U-266. Lymphoblast plasmacytoma established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968. Cell Type: B cell.
What is 4D Nucleofector?
The 4D-Nucleofector® X Unit is one of the four functional modules of the 4D-Nucleofector® System. It supports Nucleofection of various cell numbers (2 x 104 to 2 x 107) cells in different formats. There are cell type-specific Optimized Protocols or recommendations available in our knowledge database.
What is flow electroporation?
Flow electroporation (EP) permits cells to be electroporated while they flow between two electrodes and a transient electrical field is experienced by the cells as they pass between these electrodes.
How do you get gRNA sequences and Cas9 into cells?
The process first involves packaging the gRNA/Cas9 sequences into viral particles and then introducing the particles into target cells. To make the viral particles, the plasmid containing the gRNA or Cas9 sequence and plasmids containing viral genes are introduced into a packaging cell line (e.g., 293 T cells).
How does Lipofectamine work for transfection?
It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection. Lipofectamine contains lipid subunits that can form liposomes in an aqueous environment, which entrap the transfection payload, e.g. DNA plasmids.