What is the NcoI restriction site?
Thermo Scientific NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I) . See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
What is a recognition site for a restriction enzyme?
Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.
At what site does restriction enzyme HpaII cut?
Thermo Scientific HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Where is restriction enzyme found?
To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix. These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.
What is NcoI restriction enzyme?
NcoI (MB066) Description: NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by dam methylation, dcm methylation or CpG methylation. NZYTech’s NcoI is an enzyme that acts at 37 °C.
Are restriction sites palindromic?
Restriction-modification systems are used as a defensive mechanism against inappropriate invasion of foreign DNA. The recognition sequences for the common type II restriction enzymes and their corresponding methylases are usually palindromes.
What is the recognition site for HpaI?
Thermo Scientific KspAI (HpaI) restriction enzyme recognizes GTT^AAC sites and cuts best at 37°C in B buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Are restriction enzymes found in humans?
The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and nuclear extract. It proves to be an unusual enzyme, clearly related functionally to Type II endonuclease.
How are restriction enzyme sites mapped on DNA?
Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins called restriction enzymes, which can cut, or digest, DNA molecules at short, specific sequences called restriction sites.