What is the closest excitation wavelength for NHS rhodamine?
The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (“EC”; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol)….Frequently asked questions (FAQs)
| Dye | Rhodamine B |
|---|---|
| Excitation | 568 |
| Emission | 583 |
| EC | 88,000 |
What are the peak excitation and emission wavelengths of Tetramethylrhodamine?
TRITC has an absorption and excitation maximum at 550 nm (Figs 12.3 and 12.4), and an emission maximum at 580 nm.
What does TRITC stain for?
Tetramethylrhodamine (TRITC) dashboard Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.
Is rhodamine a Fluorochrome?
Principle. The fluorochrome dye, auramine-rhodamine, forms a complex with mycolic acids found in the acid-fast cell wall of organisms which resist decolorization by acid-alcohol.
How is tetramethylrhodamine used in cellular imaging?
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates,…
Which is a better reactive dye than tetramethylrhodamine?
High-performance alternatives to tetramethylrhodamine (TRITC) Reactive dyes and conjugates. The Invitrogen Alexa Fluor 555 dye—with spectral properties and quantum yield nearly identical to those of tetramethylrhodamine (TRITC) —produces brighter, more photostable conjugates.
Where are reactive groups located in Rhodamine dyes?
Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.
Where does tetramethylrhodamine accumulate in the mitochondria?
Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal is bright.