What is pET28?
Studier and co-workers1,2 described the first pET expression plasmid more than thirty years ago. pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator sequence that is included to suppress uninduced expression5.
What is pET system?
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals, expression is induced by providing a source of T7 RNA …
What is the copy number of pET28a?
(Plasmid #25630)
What are pET vectors?
pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.
What is the purpose of f1 origin in pet28 vector?
I am using pET28a vector for protein expression. This vector is containing two type of origin of replication i.e pBR322 Ori and f1 Ori. The f1 is the bacteriophage origin of replication which initiates a “rolling circle” replication in phage-infected bacteria.
What is a high copy number plasmid?
In cellular biology, the plasmid copy number is the number of copies of a given plasmid in a cell. If a plasmid has too high of a copy number, they may excessively burden their host by occupying too much cellular machinery and using too much energy.