What is Lipofectamine Plus reagent?
Lipofectamine LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities.
What is plus reagent for in transfection?
PLUS™ Reagent is a proprietary reagent for pre-complexing DNA that enhances cationic lipid-mediated transfection of DNA into many cultured eukaryotic cells.
How do you transfect HEK293 cells?
The protocol for a 24-well transfection reaction with HEK293 cells is here:
- Plate 10,000-15,000 HEK293 cells per well in 0.5 ml of complete growth medium 12-24 hours prior to transfection.
- Wash with 1xPBS and add 0.5 ml of fresh growth medium.
How do you calculate transfection efficiency?
Transfection efficiency can be calculated by counting the number of cells transfected over the total cells in a particular sample. The number of cells can be counted by two methods. The most frequently used method is to use easily tractable reporters.
Is Lipofectamine 2000 toxic to cells?
I’ve also observed toxicity with lipofectamine 2000 (as well as LTX but lower). I have found that in high concentrations, lipofectamine is toxic to HeLa cells, and as previously mentioned, the cell number seeded has an impact on this.
What is the best transfection reagent for HEK293?
Conclusion: Lipofectamine 3000 is the best choice for transfection of CHO-K1 and HEK293 with pCDH while Turbofect is preferably used in transfecting these cell lines with pEGFP-N1 (Tab.
Can you use Lipofectamine 2000 as a transfection reagent?
Lipofectamine 2000 transfection reagent works effectively with all common cell lines (Figure 2) as well as with many challenging ones and can be used in media with or without serum. View the protocols in the “resources” section below. Figure 2. High GFP expression following transfection using Lipofectamine 2000 reagent.
How is plus reagent used to improve transfection efficiency?
PLUS™ Reagent is used in conjunction with various transfection reagents, such as Lipofectamine™ Reagent, to improve transfection efficiency in adherent cell lines. Some customers find that when using PLUS™ Reagent, less optimization of DNA concentration is needed to achieve high transfection performance.
Which is the best Lipofectamine for plasmid DNA?
Lipofectamine 2000 Transfection Reagent is best transfection reagent available from Life Technologies for the co-transfection of siRNA and plasmid DNA. Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression Superior performance for co-transfection of siRNA and plasmid DNA
Which is best Lipofectamine or rnaimax for transfection?
Lipofectamine RNAiMAX works best at confluencies between 60 and 80%. Passage number may affect transfection experiments. We recommend consistent splitting and plating of cells. Excessive numbers of passages may decrease transfection performance. We do not recommend splitting cells for more than 20-30 passages.