What is an immunochromatographic assay?
The immunochromatographic assay is a simple and rapid test which represents a suitable alternative to the conventional subculture method for the primary identification of Mycobacterium tuberculosis complex in liquid cultures of BacT/Alert automated system.
What is qualitative immunoassay?
Most lateral flow immunoassays are qualitative tests, which means that a color change on the test line indicates a positive result while the lack of color indicates a negative result.
What is qualitative Immunochromatography?
Immunochromatography (or lateral flow tests) combines separation of the sample molecules and reagents based on migration on a solid support by capillary flow. The identification and detection procedures are based on the antigen–antibody immune reaction.
What is the principle of Immunochromatography?
Principle of the immunochromatographic strip test The colloidal gold test strip is based on a specific antigen–antibody reaction and detects a specific substance. An antibody is combined with gold nanoparticles by electrostatic adsorption to form the gold-labeled mAb.
What are ELISA assays?
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.
How do heterogeneous assays differ from homogenous assays?
As in a competitive, homogeneous immunoassay, unlabelled analyte in a sample competes with labelled analyte to bind an antibody. In the heterogeneous assays, the labelled, unbound analyte is separated or washed away, and the remaining labelled, bound analyte is measured.
What is involved in the reaction or in the immunochromatographic assay?
Immunochromatographic tests rely principally on the capture of the target antigen (or sometimes antibodies) from various specimens. The assay utilizes antibodies mounted on a paper strip or a nitrocellulose membrane as the immobile capture antibody (test area).
What are immunological assays used for?
The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum.