What is TrypLE select?
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.
What is the difference between TrypLE Express and TrypLE select?
Firstly, the difference between TrypLE Express and TrypLE Select is that Select is manufactured on animal origin free (AOF) machinery. TrypLE Express is also AOF but not manufactured on AOF machinery. Express and Select are both a single recombinant enzyme.
How much TrypLE should I use?
Add an appropriate volume of pre-warmed TrypLE™ Select or TrypLE™ Express to the flask (i.e., 1 mL in a T25 cm2 flask, 3 mL in a T75 cm2 flask).
How does TrypLE work?
TrypLE™ Express is a recombinant fungal trypsin-like protease, which has proven effective at dis- sociating many different attachment dependent mammalian cell lines. This tem- perature stability will allow more convenient storage and handling of this cell dissociation enzyme during routine cell culture procedures.
How do you dilute TrypLE?
TrypLE™ 10X can be used at the 10X concentration or diluted as desired for the dissociation of general cell lines from plasticware. Dilute TrypLE™ 10X as follows: 1. Prepare a 100 mM EDTA pH 8.0 (100X) solution.
How much TrypLE do I add?
Add 5–10 mL of pre-warmed complete medium to flask. Tilt flask in all directions to thoroughly rinse flask. cell suspension to a 15-mL conical tube.
How much does it cost to seed a 6 well plate?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Seeding density* | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 0.3 x 106 |
| 12-well | 150628 | 0.1 x 106 |
| 24-well | 142475 | 0.05 x 106 |
How many cells can a 6 well plate hold?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 1.2 x 106 |
| 12-well | 150628 | 0.5 x 106 |
| 24-well | 142475 | 0.24 x 106 |
How many cells per well does a 6 well plate have?
G-Rex®6 Well Plate – P/N 80240M – $98.68 Each well can expand 5 million cells into between 200 and 400 million cells in about 10 days with only one exchange of medium.
How many cells are in a 10cm plate?
100% confluent 10-cm dish contains 8.8 million HeLa cells according to some published data.