What is RF1 and RF2?
RF1 and RF2 bind to the ribosome in the space between the small and large ribosomal subunits. RF1 and RF2 differ in their stop codon specificity: RF1 utilizes a conserved PET motif to recognize UAG and UAA codons, whereas RF2 uses an SPF motif to recognize UGA and UAA codons.
What is the role of RF3 in the termination of translation?
Thus, RF3-mediated subunit rotation plays a dual role during termination, namely, to dislodge the decoding release factors from the ribosome, but also to facilitate dissociation of RF3 itself.
How do release factors work?
A release factor is a protein that allows for the termination of translation by recognizing the termination codon or stop codon in an mRNA sequence. They are named so because they release new peptides from the ribosome.
What is the function of the release factor during translation in eukaryotes?
What is the function of the release factor during translation in eukaryotes? It releases the ribosome from the ER to allow polypeptides into the cytosol. It supplies a source of energy for termination of translation. It binds to the stop codon in the A site in place of a tRNA.
What is the function of RF2?
The majority of eubacteria use two peptide release factors, release factor 1 (RF1) and release factor 2 (RF2), for recognition of translation termination codons. RF1 mediates termination at UAG and UAA, and RF2 mediates termination at UAA and UGA.
What does RF1 do in translation?
RF1 and RF2 are essential proteins that perform the well-defined function of recognizing one of three nearly universal stop codons in the ribosomal A site and catalyzing the release of the polypeptide attached to the peptidyl-tRNA in the P site.
What happens when the release factor binds?
Release factors fall into two classes; Class I release factors that bind the ribosome in response to the presence of a stop codon within the ribosomal A-site (acceptor site). This binding event triggers release of the nascent polypeptide by the ribosome, a so-called peptidyl release reaction.
What is the function of the release factor RF )?
The decoding release factor (RF) triggers termination of protein synthesis by functionally mimicking a tRNA to span the decoding centre and the peptidyl transferase centre (PTC) of the ribosome.
What happens when the release factor is placed on the mRNA molecule?
The release factors bind to a termination codon at the A site and stimulate hydrolysis of the bond between the tRNA and the polypeptide chain at the P site, resulting in release of the completed polypeptide from the ribosome. The tRNA is then released, and the ribosomal subunits and the mRNA template dissociate.
What is the final factor in eukaryotes that releases the peptide and ribosome?
RF3
What is the final factor in eukaryotes that releases the peptide and ribosome? Explanation: The RF3 is responsible for cleavage of the peptide bond as well as for the release of the ribosomal subunits in eukaryotes.