What is pet28b?
pET-28b(+) Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site.
What does pET plasmid mean?
pET plasmid (plural pET plasmids) Any of a family of vectors for protein expression where the expression of the insert DNA is controlled by the T7 promoter, a phage promoter.
How do I view SnapGene files?
Click menu File → Open File and browse to the location of the trace file, or. Drag and drop a trace file into the SnapGene Launch window, or. Double-click a trace file.
Why do we use plasmid as a vector?
Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance. Scientists have taken advantage of plasmids to use them as tools to clone, transfer, and manipulate genes. Plasmids that are used experimentally for these purposes are called vectors.
How does the pET expression system work?
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals, expression is induced by providing a source of T7 RNA …
What is pUC18 plasmid?
$ 40.00. pUC18 is a commonly used plasmid cloning vector in E. coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments.
How do I align my SnapGene?
In order to align sequences in SnapGene you should open your sequence and then select “Tools”-”Align Multiple Sequences” in the main menu (Figure 3.4. 10.1). Alternatively, press the “Show Alignment” button from the main toolbar (Figure 3.4.
What does pet 28A ( + ) DNA-Novagen carry?
More>> Novagen’s pET-28a-c (+) vectors carry an N-terminal His•Tag ® /thrombin/T7•Tag ® configuration plus an optional C-terminal His•Tag sequence. Less<< pET-28b (+) DNA – Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Is the his • tag sequence reversed in pet-28a-c ( + )?
The pET-28a-c (+) vectors carry an N-terminal His•Tag ® /thrombin/T7•Tag ® configuration plus an optional C-terminal His•Tag sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map ( TB074 ).
What kind of DNA is used in pet vectors?
The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information. This product is sold for internal research use only.
How does the f1 origin of pet work?
The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock).