What is Geneticin?
Commonly know as G418 or G-418, Gibco Geneticin reagent is an aminoglycoside related to gentamicin, and is commonly used as a selective agent for eukaryotic cells. As an analog of neomycin sulfate, it interferes with the function of 80S ribosomes and protein synthesis in eukaryotic cells.
How does geneticin work?
G418 (Geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3′-phosphotransferase, APT 3′ II.
How do I reconstitute G418?
Stock Solution Guideline: Reconstitute aseptically by adding the powder to distilled water (pH 5.6 to 7.0) to the desired concentration and sterile filter. NOTE: For G418, units/mg is exchangeable with µg/mg.
How do you prepare geneticin?
200 mg/ml G-418 Stock Solution:
- Weight 2gr of G418 (CAS: 108321-42-2 mw=692.71).
- Add 10ml of sterile H2O.
- Prewet a 0.22um syringe filter by drawing through 5-10ml pf sterile H2O and discard water.
- Sterilize G418 Stock through through the prepared 0.22um syringe filter.
- Stock may be kept at -20C for 1 year.
Is geneticin same as gentamicin?
Geneticin is a 2-deoxystreptamine antibiotic that is structurally related to gentamicin. Although not used clinically, it is employed as an antibiotic in molecular genetics experiments. Geneticin is toxic to bacteria, yeast, higher plant and mammalian cells, protozoans, and helminths.
How does Geneticin inhibit cell growth?
G-418, otherwise known as Geneticin®, is produced by Micromonospora rhodorangea through fermentation processes. And it works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells. plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3′-phosphotransferase.
How does puromycin selection work?
This compound works by interrupting protein synthesis in both prokaryotic and eukaryotic cells. This causes cells to die, unless they have a gene for resistance, allowing them to survive exposure to the antibiotic.
How does geneticin inhibit cell growth?
What is the concentration of G418 for selection?
400 to 1000 µg/mL
The concentration range of G418 that is commonly used ranges from 400 to 1000 µg/mL for selection of mammalian cells and 200 to 300 µg/mL for maintenance.
What kind of cell culture media is RPMI 1640?
Gibco Cell Culture Media. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins.
What is the GIBCO human plasma like medium ( HPLM )?
Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Select the right Gibco RPMI 1640 for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:
What kind of vitamins are in RPMI 1640 medium?
RPMI 1640 Medium contains biotin, vitamin B 12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium.