What are HB101 cells?
HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.
What is HB101 bacteria?
HB101 strain is a hybrid K12 x B bacterium, containing the recA13 mutation that minimizes recombination and aidsin insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes.
What is E coli JM109?
JM109 competent cells are a classic E. coli strain for routine cloning and plasmid maintenance. E. coli K12 JM109 is recombination- and endonuclease-deficient, improving insert stability and quality of miniprep DNA, respectively. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system.
Is hb101 a fertilizer?
HB-101 is neither an agricultural chemical nor a fertilizer. It is an all-natural solution that supports healthy plants. Hence, HB-101 is referred to as a plant vitalizer, or plant growth enhancer.
How do you read e coli genotype?
Genes: In E. coli, the genotype only includes the genes that carry a loss of function mutation. The gene name is listed as three-letters in lower case and italics (DNA methylase is written as dam). Different genes affecting the same function/pathway are identified with different uppercase italic letters.
Is HB-101 a fertilizer?
Is E coli HB-101 Gram positive or negative?
coli are gram-negative, rod shaped bacteria that were named after Dr. Theodor Escherich, the scientist who first described them in 1885. E. coli are mainly found in the intestinal tract of animals.
What genotype is E coli JM109 Promega?
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
What is HB-101 used for?
E. coli HB101 Competent Cells can be used for preparation of a DNA library or subcloning of a recombinant plasmid.
How often can I use HB-101?
How often should you apply HB101? For our indoor plants, HB-101 is best used at a maintenance dose every time you water (typically weekly to fortnightly in warmer months, then 3 to 4 weekly in winter depending on your plant’s water requirements).
What is the genotype of E. coli?
Genotypes of Invitrogen competent cells
| Bacterial strain | Genotype |
|---|---|
| DH10B | F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG |
| DH10B T1R | F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG tonA |