What are exogenous proteins?
The term exogenous protein metabolism implies the breakdown of food proteins which do not exist as parts of the cell protoplasm. The replacement of protein is rapid in plasma, liver, kidneys and intestinal tract and slow in haemoglobin, muscle and skin.
What is endogenous expression?
To estimate the difference in the timing of expression, Takashima et al. [7] compared the expression patterns of the luciferase reporters with the pattern of the Hes7 transcript and the endogenous Hes7 protein in the PSM.
What is LgBiT protein?
The intracellular LgBiT expression reagents allow HiBiT-tagged proteins to be used as the bioluminescent energy donor in live-cell NanoBRET™ assays for the study of protein:protein interactions or small molecule target engagement.
What is a HiBiT assay?
Detect low-abundance proteins The HiBiT assay is based on bioluminescence, which is more sensitive than fluorophore-based methods, such as GFP. This allows detection at extremely low levels. Not only is it sensitive, it is also highly quantitative.
What is the consequence of excess protein intake?
Extra protein is not used efficiently by the body and may impose a metabolic burden on the bones, kidneys, and liver. Moreover, high-protein/high-meat diets may also be associated with increased risk for coronary heart disease due to intakes of saturated fat and cholesterol or even cancer [31].
What is the difference between endogenous and exogenous sources?
In an economic model, an exogenous variable is one whose value is determined outside the model and is imposed on the model, and an exogenous change is a change in an exogenous variable. In contrast, an endogenous variable is a variable whose value is determined by the model.
Why is gene expression important?
Genes encode proteins and proteins dictate cell function. Therefore, the thousands of genes expressed in a particular cell determine what that cell can do. Thus, control of these processes plays a critical role in determining what proteins are present in a cell and in what amounts. …
Why is gene regulation important?
Gene regulation is an important part of normal development. Genes are turned on and off in different patterns during development to make a brain cell look and act different from a liver cell or a muscle cell, for example. Gene regulation also allows cells to react quickly to changes in their environments.
What is a NanoBiT assay?
The NanoBit ® assay provides a tool for detecting protein-protein interactions in live cells ( Figure 1A). The assay is based on splitting the engineered luminescent protein NanoLuc ® into two separate subunits, the small BiT (SmBiT, 1.3 kDa in size) and the Large BiT (LgBiT, 18 kDa in size).
What is a Bret assay?
BRET, or Bioluminescence Resonance Energy Transfer, is a cell-based assay for studying protein-protein interactions. BRET relies on Forster resonance energy transfer from a bioluminescent donor to an acceptor in the presence of a substrate. Interestingly, BRET is a naturally occurring phenomenon in sea pansy.
What does Hibit stand for?
HIBIT
| Acronym | Definition |
|---|---|
| HIBIT | Health Informatics and Bioinformatics, Turkey (symposium) |
What is the name of the technology #promega has developed for protein tagging?
HiBiT Protein Tagging System
–(BUSINESS WIRE)–Promega Corporation announced today that its HiBiT Protein Tagging System has been listed as a 2017 Top 10 Innovation by life science publication The Scientist.