Which stain is used for peripheral blood smear?
Commonly used stain in our environment is Leishman stain which is composed of polychrome methylene blue (basic component) and eosin (acidic component). May-Grunwald Giemsa or Wright-Giemsa stain can also be used.
Can you see bacteria in blood smear?
It is unusual to find microorganisms in peripheral blood smears, and their presence is frequently associated with overwhelming sepsis and consequently a poor prognosis. In this report, we demonstrate 4 cases with bacteria in blood smears.
What is the procedure for staining a blood smear using Wright’s stain?
Procedure
- Prepare a film of blood or bone marrow on a microscopic slide and allow to air dry.
- Place the air-dried smear on the slide staining rack, smear side facing upwards.
- Cover the blood film with undiluted staining solution.
- Let stand for 2-3 minutes.
- Add approximately equal amount of buffered water (pH 6.5).
How do you stain a smear?
- Smear is fixed in methanol.
- Stain the film with dilute dye solution (1:10) for 5 minutes.
- Then stain with dilute Giemsa’s stain (1:10) for 10 to 15 minutes.
- Wash with buffered water and dry in the air.
What is the purpose of applying a stain to a bacterial smear?
What is the purpose of applying a stain to a bacterial smear? To provide contrast between the organism and the background. Since bacterial cells are generally transparent, it is very helpful to stain bacteria to provide contrast.
Why is peripheral smear test done?
A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.
How do you do a Wrights stain?
Wright Stain Method
- Place 1.0 ml of the Wright Stain Solution upon the smear 1 – 3 minutes.
- Add 2.0 ml distilled water or Phosphate buffer pH 6.5 and let stand twice as long as in step 1.
- Rinse stained smear with water or the Phosphate buffer pH 6.5 until the edges show faintly pinkish-red.
What is Wright stain procedure?
PRINCIPLE: Wright’s stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue. When applied to blood cells, the dyes produce multiple colors based on the ionic charge of the stain and the various components of the cell.