What is the unit of the slope of the Lineweaver-Burk plot?
The Lineweaver-Burk plot results in a straight line with the slope equal to KM/k2[E]0 and y-intercept equal to 1/k2[E]0 which is 1/Vmax via Equation 10.2.
What is the slope in Michaelis Menten?
The slope of the line is Km/Vmax, and the y-intercept is 1/Vmax.
What is Lineweaver-Burk plot equation?
In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.
What is Lineweaver-Burk equation?
The Lineweaver-Burk equation is a linear equation, where 1/V is a linear function of 1/[S] instead of V being a rational function of [S]. The Lineweaver-Burk equation can be readily represented graphically to determine the values of Km and Vmax. Given a Lineweaver-Burk plot, determine the Vmax of a particular enzyme.
What is the Michaelis Menten equation and its Lineweaver-Burk form?
8.3. In the line weaver Burk (LB) plot the Michaelis Menten equation is converted into straight line curve. It is used to estimate the Vmax from the position of intercept on the X-axis. Straight line is given by Y = MX + C, where C is Y intercept of the regression of Y on X and M is slope.
How do you find slope in economics?
The slope of a line is determined by taking the change in the vertical amount divided by the change in the horizontal amount. We will let the Greek symbol Delta represent the change. In our example, as x increases by 2, y increases by 4 so the slope would a positive 2.
How is the slope of a Lineweaver-Burk plot determined?
It gives a straight line, with the intercept on the y-axis equal to 1/Vmax, and the intercept on the x-axis equal to Km/Vmax. The slope of the line is equal to Km/Vmax. Vmax and Km can be determined experimentally by measuring V0 at different substrate concentrations. Then a double reciprocal or Lineweaver–Burk plot of 1/V0 against 1/ [S] is made.
Who is the author of the Lineweaver-Burk plot?
The Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.
Can a double reciprocal Lineweaver – Burk plot be made?
Then a double reciprocal or Lineweaver–Burk plot of 1/V0 against 1/ [S] is made. Reversible enzyme inhibitors can be classified as either competitive or noncompetitive, and can be distinguished via a Lineweaver–Burk plot.
How is competitive inhibition measured in a Lineweaver-Burk plot?
Competitive inhibition can be recognized by using a Lineweaver–Burk plot if V0 is measured at different substrate concentrations in the presence of a fixed concentration of inhibitor. A competitive inhibitor increases the slope of the line on the Lineweaver–Burk plot, and alters the intercept on the x-axis (since Km is increased),