What is the role of the nuclear localization sequence?
A nuclear localization signal or sequence (NLS) is an amino acid sequence that ‘tags’ a protein for import into the cell nucleus by nuclear transport. An NLS has the opposite function of a nuclear export signal (NES), which targets proteins out of the nucleus.
How does targeted sequencing work?
Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest. This method generally requires less sample input and produces a smaller amount of data than WGS, making analyses more manageable.
What is a target sequence?
Targeted sequencing is a rapid and cost-effective way to detect known and novel variants in selected sets of genes or genomic regions. Gene sequencing can be accomplished using several different DNA sequencing methods, depending on the scale.
What is target sequence capture?
Targeted capture or target enrichment allows for simultaneous sequencing of sets of genomic regions of interest in several individuals in the same sequencing run, therefore reducing sequencing costs. This technique uses custom RNA probes which are complementary to the target genomic regions.
What property do nuclear localization signal sequences have?
D Nuclear localization signal (NLS) sequences NLS sequences are typically short peptide sequences responsible for direct import of proteins into the nucleus. In general, these sequences contain a high proportion of the basic amino acids lysine and arginine (Lanford and Butel, 1984).
What is targeted variant analysis?
Summary. Confirmatory analysis of research or results reported by another laboratory. Testing of published founder or common variants. Clarification of variant classification for variants reported by another laboratory.
What is targeted mutation?
Targeted Mutation. MGI Glossary. Definition. A type of mutation in which a chromosomal gene is altered by the substitution of a DNA construct assembled in vitro. In mouse, the constructs are usually designed to eliminate gene function; such targeted mutations are often casually referred to as knock outs.
How long is a target sequence?
known. Typically, this target sequence ranges from 100 to 1,000 base pairs in length. To per- form PCR, two small oligonucleotide primer sequences must be synthesized that typically are 16 to 20 base pairs in length. These primer sequences are complementary to the 3′ ends of the sequence to be amplified (Fig 1).
What is sequence capture?
Sequence capture technology allows targeted enrichment of specific regions of a genome such as an entire exome. This, in concert with NGS, provides an efficient strategy for high-throughput screening of regions of interest, facilitating the identification and characterisation of physiologically relevant variants.
What usually happens to the signal sequence in a nuclear protein Once the protein has been transported into the nucleus?
the signal sequence remains bound to the channel while the rest of the polypeptide is threaded through the membrane. how does the ribosome synthesize a protein destined for the ER?