What is the mode of action of acridine dye?
Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein.
How does acridine orange work?
Acridine orange is a dye that intercalates or binds with the nucleic acid ( either DNA or RNA) present in organisms and fluoresce to emit various colors that help in the differentiation of cellular organelles.
What type of stain is acridine orange?
Acridine Orange (AO) is a nucleic acid selective metachromatic stain useful for cell cycle determination. AO interacts with DNA and RNA by intercalation or electrostatic attraction respectively.
How does acridine orange bind?
Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as a lysosomal dye.
Where is acridine orange applied?
Acridine orange as a fluorescent stain has been extensively used in the differential staining of a variety of materials within tissue and other mediums. One especially advantageous application has been in the demonstration of fungi.
Is acridine orange chemical mutagen?
One of the first uses of reversion tests was to determine how various chemicals induce mutations. For example, acridine dyes are mutagenic.
How acridine orange causes carcinogenesis?
The photodamage caused by AO was nearly neglected in SV-Huc-1 cells, suggesting a differential effect of this treatment between cancer and normal cells. In summary, AO, as a photosensitizer, disrupts acidic organelles and induces cancer cell death in BC cells under blue-light irradiation.
Is acridine an orange mutagen?
Acridine orange (AO) and methylene blue (MB) in the dark were shown to be weak to moderate mutagens (induction of resistance to T5 phage) in repair-deficient strains of Escherichia coli B/r. However, strain WP2 (wild-type) was not mutated by AO in the dark, in confirmation of earlier data.
Is acridine orange soluble in water?
This product is soluble in water (6 mg/ml), in ethanol (2 mg/ml), and in 2-methoxyethanol (EGME, 4 mg/ml). 1 At 1 mg/mL in water, it forms a clear, dark orange to amber solution. 1. The Sigma-Aldrich Handbook of Stains, Dyes & Indicators, Green, F.J., Ed., Aldrich Chemical Co.
What is the principle of acridine orange staining?
Principle : Acridine orange is a cell-permeable,nucleic acid selective dye that emits green fluorescence when bound to dsDNA (at 520 ) and red fluorescence when bound to ssDNA or RNA (at 650 nm).Since it is a cationic dye,it also enter acidic compartments such as lysosomes which in low pH conditions, will emit orange light.
What kind of drug is acridine orange used for?
Acridine orange (AO) is a common fluorescent dye that has been well known for years. It has recently regained attention as a possible innovative drug for clinical applications in the field of oncology, particularly in cancer imaging and photodynamic therapy.
How is acridine orange similar to fluorescein?
Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein. Acridine orange and fluorescein have a maximum excitation at 502 nm and 525 nm (green).
How are acridine oranges related to xanthene molecules?
Acridine orange (AO) is a member of the xanthene class of molecules and shares a common aromatic structure with multiple acridine dyes (1, 2). Acridine dyes were first extracted from coal tar at the end of the nineteenth century. And was then used for a period of time as a dye in the fabric industry as well as for biological application.