What is lateral flow immunoassay procedure?
So, what is a lateral flow immunoassay? Basically, it is a simple to use diagnostic device used to confirm the presence or absence of a target analyte, such as pathogens or biomarkers in humans or animals, or contaminants in water supplies, foodstuffs, or animal feeds.
What is a lateral flow test for coronavirus?
Rapid lateral flow tests help to find cases in people who may have no symptoms but are still infectious and can give the virus to others. The test usually involves taking a sample from your throat and nose, or from your nose only, using a swab.
How is lateral flow test done?
open your mouth wide and rub the swab over your tonsils (or where they would have been). Avoid the end of the swab touching your teeth, tongue and gums. put the same swab inside your nose (about 2.5 cm up or until you feel some resistance)
What is Immunochromatography method?
Immunochromatographic technique. Immunochromatography is a testing method for detecting a disease by dropping the sample containing an analyte onto a test strip. This is a speedy and simple technique that produces diagnostic results in 10 to 15 minutes after dropping the sample.
How does a lateral flow test work in immunochromatography?
The identification and detection procedures are based on the antigen–antibody immune reaction. A typical lateral flow test format usually consists of a nitrocellulose or filter paper membrane that carries the liquid sample from the application pad via the conjugate release pad (e.g., lyophilized labeled antibodies), along a strip.
What is lateral flow test and what does it mean?
This method is also known as the Lateral Flow Test and provides a simple and fast way to test a liquid patient sample, such as blood, urine, serum, saliva, sweat or other fluids, for a specific analyte (Koczula & Gallotta, 2016 ).
When to use a lateral flow assay ( LFA )?
The lateral flow assay (LFA) is a paper-based platform for the detection and quantification of analytes in complex mixtures, where the sample is placed on a test device and the results are displayed within 5–30 min.
What is the principle of lateral flow immunoassay?
Principle of the lateral flow immunoassay The principle behind the LFA is simple: a liquid sample (or its extract) containing the analyte of interest moves without the assistance of external forces (capillary action) through various zones of polymeric strips, on which molecules that can interact with the analyte are attached.