What is desmin staining?
Desmin is a cytoplasmic stain and with formalin-fixed, paraffin-embedded tissue will identify almost 80% of all RMSs.
Where is desmin found?
Desmin filaments are mainly located at the periphery of Z-disk of striated muscles and at the dense bodies of smooth muscle cells, and they have been postulated to play a critical role in the maintenance of structural and mechanical integrity of the contractile apparatus in muscle tissues.
What is desmin cardiomyopathy?
Mutations in the desmin gene affecting intermediate filaments cause a distinct myopathy that is often associated with cardiomyopathy and is termed “desmin myopathy.” The mutant desmin interferes with the normal assembly of intermediate filaments, resulting in fragility of the myofibrils and severe dysfunction of …
What is desmin a marker of?
Desmin is a specific marker for rhabdomyosarcomas of human and rat origin.
Is leiomyosarcoma desmin positive?
Leiomyosarcoma shows easily identifiable mitotic figures in addition to nuclear atypia and cellular pleomorphism. Leiomyosarcoma shows strong positive reaction in tumor cells for smooth muscle actin. Leiomyosarcoma shows strong positive reaction in tumor cells for desmin.
What does HMB 45 stain?
HMB-45 is a widely used immunohistochemical stain for detection of primary as well as metastatic melanoma (1). Presumably, this method uses monoclonal antibodies to a glycoprotein that is present in premelanosomes (11).
What would result if the protein desmin degrades in muscle?
The degradation of desmin, which plays a role in linking adjacent myofibrils together, may cause physical changes to the postmortem muscle. Thus, the degradation of desmin and the connecting intermediate filaments may play an important role in meat tenderness during aging (Yamaguchi et al., 1982, 1983a, b).
What causes desmin myopathy?
Desmin myopathy is associated with mutations in desmin, αB‐crystallin and perhaps other genes interacting with desmin. Disease‐causing desmin mutations affect amino‐acid residues at sites that are critical for filament assembly.
What is Helmer’s myopathy?
Desmin-related myofibrillar myopathy, also called Helmer’s myopathy, is a subgroup of the myofibrillar myopathy diseases and is the result of a mutation in the gene that codes for desmin which prevents it from forming protein filaments, instead forming aggregates of desmin and other proteins throughout the cell.
What are the positive control cells for desmin?
Positive Control: Skeletal and cardiac muscle cells, smooth muscle cells of blood vessels, viscera, the uterus as well as in Purkinje cells, many mesangial cells of the kidney glomeruli, leiomyoma and rhabdomyomas.
Where does desmin come from in the body?
Desmin is a protein made by muscle cells. It is produced by both normal muscle cells and tumours made out of muscle. Cardiac muscle cells – These are the cells that make up the heart.
How is desmin measured in a pathology report?
Pathologists perform a test called immunohistochemistry to look for cells producing desmin. This test helps your pathologist decide if the tumour is made up of muscle cells or if the cells are starting to behave like muscle cells. If the cells in your sample produce desmin, your pathology report will describe the cells as positive or reactive.