What CellROX detects?
CellROX® Green Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells.
What is CellROX?
We are Cellrox. Experienced in the cell phone accessories and repair service business since 2002, we are currently operating Kiosks, Carts and Stores in various malls across Canada.
How does CellROX work?
CellROX Green Reagent is a nucleic acid binding dye, and upon oxidation, it binds predominantly to DNA and thus, its signal is localized primarily in the nucleus and mitochondria. The staining workflow is simple and the reagent can be applied to cells in complete growth media or buffer.
What are the three types of ROS?
Reactive oxygen species (ROS), such as superoxide anion (O2−), hydrogen peroxide (H2O2), and hydroxyl radical (HO•), consist of radical and non-radical oxygen species formed by the partial reduction of oxygen.
What Amplex Red?
Additional Information. The Amplex Red reagent, a highly sensitive and stable probe for H2O2, is our best fluorogenic substrate for peroxidase. In the presence of horseradish peroxidase (HRP), the Amplex Red reagent reacts in a 1:1 stoichiometry with H2O2 to produce highly fluorescent resorufin.
Is ROS good or bad?
ROS are predominantly beneficial to cells, supporting basic cellular processes and viability, and oxidative stress is only an outcome of a deliberate activation of a physiological cell death pathway. Maintaining a basal level of ROS in cells is essential for life.
How does ROS cause cell death?
ROS/RNS can cause cell death by nonphysiological (necrotic) or regulated pathways (apoptotic). The mechanisms by which ROS/RNS cause or regulate apoptosis typically include receptor activation, caspase activation, Bcl-2 family proteins, and mitochondrial dysfunction.
Is hydrogen peroxide a ROS?
Among the ROS generated are hydrogen peroxide (H2O2), hypochlorite (HOCl), hydroxyl, and superoxide (O2−). These oxidants can induce injury in a variety of mammalian cells, including endothelial cells (ECs). Although O2− can be directly toxic, it has limited reactivity with most biological molecules.
What is a 10 point ROS?
our physicians use this statement for ROV: “10 point review of systems is otherwise negative except as mentioned above.” Is this sufficient for a COMPLETE review of systems? No… “A complete ROS inquires about the system(s) directly related to the problem(s) identified in the HPI plus all additional body systems.
How does Amplex Red work?
Amplex Red, in the presence of peroxidase enzyme, reacts with H2O2 in a 1:1 stiochiometry to produce resorufin, a red fluorescent compound (Figure 1) which has an absorption and fluorescence emission maxima of 563 nm and 587 nm respectively [2].
How does the Cellrox deep red reagent work?
Description CellROX® Deep Red Reagent is a novel fluorogenic probe for measuring cellular oxidative stress in both live and fixed cell imaging, with absorption/emission maxima at ∼644/665 nm. The cell-permeant dye is non-fluorescent while in a reduced state, and exhibits bright fluorescence upon oxidation by reactive oxygen species (ROS).
How does the cell permeant dye in Cellrox work?
The cell-permeant dye is non-fluorescent while in a reduced state, and exhibits bright fluorescence upon oxidation by reactive oxygen species (ROS). Features of the CellROX® Deep Red Reagent: The CellROX® Deep Red reagent is a novel cell-permeant dye with absorption/emission maxima of ~644/665 nm.
How is Cellrox used to detect oxidative stress?
Oxidative Stress Detection with CellROX® Deep Red Reagent. Oxidative stress results from an imbalance between production of reactive oxygen species (ROS) and the ability of cells to scavenge them.
How are Cellrox dyes used to detect reactive oxygen species?
CellROX Dyes are non-fluorescent in a reduced state and exhibit bright, stable fluorescence upon oxidation by reactive oxygen species (ROS). They are membrane permeant and load readily into live cells