How do I connect my Bluetooth to my Volkswagen?
- Press “Setup” on the infotainment display.
- Press “Bluetooth.”
- Press “Search for devices.”
- Press “Results.” Select your device.
- You’ll have two device profile options: “Handsfree” or “Bluetooth® Audio.” Choose one.
- After confirming that the same PIN is shown on your phone, press “Yes” on the infotainment screen.
How do I connect my Polo Vivo Bluetooth?
Steps to Connect iPhone to VW Bluetooth
- In your phone’s settings make sure your Bluetooth is on.
- Make sure vehicle is turned on or in auxiliary mode (radio/lights only)
- Phone will search for Bluetooth.
- When “VW Phone” appears, select and pair.
How to activate and expand Treg cells in humans?
This protocol is intended for activation and expansion of human Treg cells isolated with the Dynal® CD4 + CD25 + Treg Kit (Cat. no. 113.23D). The expanded Treg cells retain their regulatory capacity (1-3).
What should be the purity of Treg cells?
At least 95% pure Treg cells will give the most optimal expansion results. Low purity will allow CD8 + and CD4 + CD25- T cells to grow and reduces the number of Treg cells in relative terms. If the purity is lower than recommended above, the expansion results might be sub-optimal.
When to re-stimulate Treg cells after restimulation?
Split the cultures when needed and rest the cells until Day 21-24 in culture medium with 100 U/ml rIL-2. Incubate in a humidified CO2 incubator at 37°C. The cells can be re-stimulated 15-18 days after the first stimulation, or when cell shrinking and reduced rate of proliferation is observed. Guidelines for restimulation are provided in Table 2. 1.
Is there a ready to use Treg expander?
Ready-to-use Dynabeads® Human Treg Expander offer the first artificial antigen- presenting cells to provide simultaneous signals to TCR/CD3 and CD28 for full activation and expansion of human Treg cells.