What is the purpose of selenite F?
Selenite-F Broth is used as an enrichment medium for the isolation of Salmonella from feces, urine, water, foods and other materials of sanitary importance. The casein peptone provides essential nitrogenous and carbon compounds. The lactose in the medium serves to maintain a uniform pH.
Is selenite F broth a selective differential media?
Selenite broth is used as a selective medium for the isolation of Salmonella species. Selenite broth was originated by Leifson, while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Selenite broth is used as a selective enrichment for the cultivation of Salmonella spp.
What is selenite cystine broth?
Selenite Cystine Broth is used as a selective enrichment medium for the isolation of Salmonella from feces, foods, pharmaceutical articles, water and other materials of sanitary importance. This medium conforms with specifications of The United States Pharmacopeia (USP).
What type of media is selenite?
Remel Selenite Broth w/ and w/o Cystine are liquid media recommended for use in qualitative procedures for selective enrichment of Salmonella, and to a lesser degree Shigella sonnei. Casein peptone supplies nitrogenous substances and carbon compounds required for bacterial growth.
Why is selenite broth used for Salmonella?
Selenite Broth was devised by Leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in fecal specimens and, thus, was beneficial in the recovery of Salmonella species.
What is enrichment broth?
An enrichment broth was developed for the efficient isolation of Escherichia coli O157 from radish sprouts. The broth was buffered peptone water containing 0.5% sodium thioglycolate (STG-BPW), which was designed to allow growth of E. coli O157 in starved and unstarved states.
What is the difference between nutrient agar and nutrient broth?
The main difference between nutrient agar and nutrient broth is that nutrient agar is a solid medium whereas nutrient broth is a liquid medium. Agar is added to the nutrient agar in order to solidify the medium. Nutrient agar is prepared in Petri dishes while nutrient broth is prepared in test tubes or bottles.
How do you make selenite broth?
Preparation of Selenite F Broth
- Add 4.0 gm sodium selenite powder to distilled/deionized water.
- Add remaining 19.0 gm powder to above solution and bring volume to 1.0 liter.
- Gently heat and bring to boiling.
- Dispense into sterile test tubes, at least of a depth of 5 cm.
How do you make selenite cystine broth?
Suspend 19.01 grams in 1000 ml purified / distilled water. Add 4 grams of sodium hydrogen selenite (M1079B). Warm to dissolve the medium completely. Distribute in sterile test tubes.
What is selenite F?
Selenite F Broth is the medium used for the selective enrichment of Salmonella spp from both clinical and food samples. F in Selenite F Broth is Faeces, because it is mostly used for the isolation of Salmonella and Shigella from faecal specimens.
What is an enrichment broth and why is it used?
M-Enrichment Broth is used as a non-selective medium for enumeration of bacteria by membrane filter technique and for preliminary enrichment of organisms on membrane filter prior to using selective media.