What is Butterfield phosphate buffer?
Remel Phosphate Buffer (Butterfield’s Buffer) is a liquid medium recommended for use in qualitative procedures as a diluent in microbial limit testing of pharmaceutical products and in food testing.
How do you make phosphate buffer?
“Phosphate Buffer (pH 5.8 to 7.4) Preparation.” AAT Bioquest, Inc, 23 Nov….
- Prepare 800 mL of distilled water in a suitable container.
- Add 20.214 g of Na2HPO4•7H2O to the solution.
- Add 3.394 g of NaH2PO4•H2O to the solution.
- Adjust solution to final desired pH using HCl or NaOH.
What are the components of phosphate buffer?
Phosphate-buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
How do you make buffered peptone water?
Suspend 20.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in 50 ml amounts into tubes or flasks or as desired. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.
How do I make a 10X PBS buffer?
To make 1 L of 10X PBS stock solution, combine 17.8 g of Na2HPO4, 2.4 g of KH2PO4, 80 g of NaCl, 2 g of KCl, and adjust final volume to 1 L. This recipe calculator enables the accurate preparation of 10X PBS for any millimeter volume.
How do you make a 6.8 phosphate buffer?
Phosphate Buffer pH 6.8, Mixed: Dissolve 28.20 g of disodium hydrogen phosphate and 11.45 g of potassium dihydrogen phosphate in sufficient water to produce 1000 ml.
How do you make 0.1 M potassium phosphate buffer?
Potassium Phosphate Buffer (1 M, pH 6.5) Preparation and Recipe
- Prepare 800 mL of distilled water in a suitable container.
- Add 95 g of KH2PO4 to the solution.
- Add 52.5 g of K2HPO4 to the solution.
- Adjust the pH to 6.5.
- Filter sterilize and store at room temperature.
How do you make 10 mM potassium phosphate buffer?
10 mM Potassium Phosphate, 100 mM Sodium Chloride, 0.1% Sodium Azide, pH 7.6 ± 0.1. 1. Dissolve Potassium Phosphate (dibasic and monobasic) and Sodium Chloride in approx. 80% of final volume with dH2O at room temperature.
How do you make 0.1 buffered peptone water?
0.1% Peptone Salt Solution is used as diluent for different test method. Suspend 9.50 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes i.e. validated cycle.
How do you make buffered sodium chloride peptone solution?
Suspend 14.63g of Sodium Chloride Peptone Broth (Buffered) in 1 litre of water (purified as required). If required, add 1-10ml Tween® 20 or 80 per litre of medium. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.
When do you use Remel Butterfield phosphate buffer?
Formulation used in food, dairy and pharmaceutical testing by AOAC, APHA, FDA, USDA, and USP test methods. Thermo Scientific™ Remel Butterfield’s 90mL Phosphate Buffer (pH 7.2 stock solution) formulation is used in food, dairy and pharmaceutical testing as referenced in AOAC, APHA, FDA, USDA, and USP test methods.
When to use phosphate buffer, pH 7.2?
Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended. This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food (2).
How long to sterilize buffered dilution water bottle?
Store in refrigerator. Take 1.25 ml of above stock solution and bring volume to 1 liter with distilled water. Dispense into bottles to 90 or 99 ± 1 ml. Sterilize 15 min at 121°C. ( Note: This same formulation is referred to as Buffered Dilution Water in American Public Health Association. 1970.
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