What is ALOA Agar?
ALOA® Agar is a selective chromogenic medium for the detection of Listeria monocytogenes and Listeria spp. in food and environmental samples. It is an AOAC-approved method for the confirmation of VIDAS® LPT, VIDAS LIS and VIDAS LMX-positive assays. ALOA agar provides reliable and rapid results in 24 hrs at 37°C.
What is Oxford agar used for?
Remel Oxford Agar is a solid medium recommended for use in qualitative procedures for the selective isolation of Listeria monocytogenes in food samples. Columbia Agar Base provides peptones and other essential nutrients.
What is the indicator used in the Palcam media?
PALCAM medium is a differential diagnostic medium utilizing two indicator systems. One system is based on the hydrolysis of esculin to esculetin and dextrose, which reacts with ferric citrate producing a brownish black precipitate around the colonies. Mannitol and phenol red is the other indicator system.
What is the selective media for Listeria monocytogenes?
A new selective medium (Al-Zoreky-Sandine listeria medium [ASLM]) was formulated to recover Listeria monocytogenes from food specimens; the medium completely inhibited common food microflora.
What Agar is used for listeria monocytogenes?
Oxford agar
Esculin based Listeria selective agars: Oxford agar (OXA) (18) ( M118). After 24 h incubation at 35° C typical Listeria species colonies are approximately 1 mm diameter, gray to black colonies surrounded by a black halo.
How do you contract listeria?
Listeria can be spread to people by several different methods. Eating food contaminated with the bacteria, such as through raw (unpasteurized) milk or contaminated vegetables, is often a source for cases. The bacteria may be passed from mother to fetus during pregnancy or directly to the newborn at the time of birth.
What Agar does Listeria grow on?
Listeria species grow on media such as Mueller-Hinton agar. Identification is enhanced if the primary cultures are done on agar containing sheep blood, because the characteristic small zone of hemolysis can be observed around and under colonies.
Does Listeria monocytogenes grow on MacConkey Agar?
Grow at/on: pH 5.5-9.6 (pH 7.2-7.6 optimum). Temperature range 0-45 ºC. Grow on 40% bile agar. Weak growth on MacConkey agar.
What is the difference between Listeria spp and Listeria monocytogenes?
The key difference between Listeria monocytogenes and Listeria spp is that L. monocytogenes is a foodborne human pathogenic species of the genus Listeria while Listeria spp is a member of the Listeria genus that contains 21 species, including pathogenic L. monocytogenes and non-pathogenic Listeria innocua.
Is the ALOA agar plate easy to read?
ALOA agar provides reliable and rapid results in 24 hrs at 37°C. The plate is easy to read with blue to blue-green colonies on an opaque background. L. monocytogenes produces a halo on the agar, Listeria spp. does not.
What does Aloa stand for in medical category?
DESCRIPTION Agar Listeria acc. to Ottaviani & Agosti (ALOA), complete with selective and enrichment supplements, is a selective and differential medium for the isolation of Listeria spp. from foodstuffs and other samples and for the identification of L. monocytogenes.
When to use ALOA to differentiate listeria ACC?
Agar Listeria acc. to Ottaviani & Agosti (ALOA) allows to differentiate L. monocytogenes even in presence of a mixed flora, after incubation of 24 +/- 2 hours in an easy and reliable way, as well as direct streaking, or after enrichment in the usual selective liquid media.