Why is DMEM red?
DMEM contains phenol red, which is solely a pH indicator. At physiological pH, the media is a pink-red color. Additionally, DMEM is a bicarbonate buffered system, which relies heavily on this CO2 concentration. If the gas in your incubator is off, this will dramatically change the pH in your media.
What is the pH of DMEM?
DMEM is buffered for high CO2. Your DMEM probably has 3.7 g sodium bicarbonate which will provide a pH of 7.4 in 10% CO2. If you remove the high CO2, the sodium bicarbonate takes over and you get a basic pH.
Is phenolphthalein a phenol?
is that phenolphthalein is (chemistry) a quinone derivative used as a ph indicator, and once used as a laxative while phenol is (organic compound|uncountable) a caustic, poisonous, white crystalline compound, c6h5oh, derived from benzene and used in resins, plastics, and pharmaceuticals and in dilute form as a …
Does DMEM contain proline?
DMEM does not contain proline.
What is the difference between DMEM and DMEM?
DMEM is modified form of EMEM, where the nutrient concentrations are increased along with the addition of some new components. EMEM is a minimal media and contains all the essential factors required for the successful growth of the animal cell lines. This is the difference between DMEM and EMEM.
Are there any phenol red media in DMEM?
We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent. What is the manganese concentration in DMEM?
Is there phenol red in live cell imaging?
Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging.
When to use GIBCO high glucose, no phenol red?
Gibco™ DMEM, high glucose, no glutamine, no phenol red Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine, No Phenol Red is used in cell culture processing applications. $30.77 – $266.00
Are there any lipids or proteins in DMEM?
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH. For Research Use Only.